Interpretation of a brain SPECT using 99mTc labeled cerebral perfusion imaging agent such as 99mTc-ECD requires high uptake of the tracer in the brain. Extracerebral soft tissue activity higher than that of cerebral activity is infrequently observed. We report two patients with extracerebral soft tissue activity when they were administered 99mTc-ECD prepared from the same kit vial. This was apparent radiopharmaceutical fault with low radiochemical purity of 99mTc-ECD.