界面活性劑生產菌應用於撚料油污染土壤之生物復育

Application of Biosurfactant Producing Bacteria for Bioremediation on Fuel Oil Contaminated Soils

林忠亮(Chung-Liang Lin) ； 黃冬梨(Tung-Li Huang)

《石油季刊》 37卷4期 (2001/12) Pp. 51-58

https://doi.org/10.30128/JP.200112.0007

摘要

自高雄某處油污染的土壤中，以集積培養或直接分離方法得到25株燃料油利用菌，其中包括有十株界面活性劑生產菌，根據其界面活性物質的特性分成表面活性劑生產菌與乳化劑生產菌二類。本研究在探討界面活性劑生產菌、營養源與通氣狀況對土壤中燃料油之陳解效應，並模擬油汙染在土壤中生物復育之可行性。研究結果顯示，添加表面活性劑生產菌群能有效促進土壤中燃料油的降解，而氧氣之供給為土壤整治成功與否之重要因子，添加適量無機鹽營養源亦可加速燃料油降解，但效果並不明顯。這些表面活性劑生產菌群應可用在油汙染場址進行土壤之生物復育。

關鍵字

油汙染 ； 生物復育 ； 界面活性劑生產菌

並列摘要

Twenty-five microbial stains were isolated from petroleum hydrocarbon-contaminated soils by enrichment method with fuel oil as the only carbon source. Ten of them possessed the capability of producing bioemulsifier and biosurfactant while grown in a mineral medium with glucose and glycerol. The bioremediation study was performed in laboratory, and the effect of bioemulsifier- and biosurfactant- producing bacteria, nutrients, and aeration of the degradation of fuel oil in soils were evaluated. The results indicated that the forced aeration was a crucial factor for a successful remediation. The fuel oil degradation was significantly enhanced when the biosurfactant-producing bacteria were inoculated. The rate also slightly increased but not significantly when nutrients were added to soils. An alternative way was suggested of which bioremediation was carried out by inoculating the biosurfactant- producing microorganisms into the hydrocarbon-contaminated soil rather than adding the biosurfactant.

並列關鍵字

Oil contaminated soils ； Bioremediation ； Biosurfactant-producing bacteria