整合生物製程生產生質酒精與生質丁醇之研究

The Study of an Integrated Bioprocess for Production of Bioethanol and Biobutanol

林忠亮(Chung-Liang Lin) ； 林士民(Shyh-Mirn Lin) ； 湯雅理(Ya-Li Tang) ； 陳文章(Wen-Chang Chen)

《石油季刊》 48卷3期 (2012/09) Pp. 53-64

https://doi.org/10.30128/JP.201209.0005

摘要

本研究利用具生產生質酒精與麩胱甘肽（Glutathione, GSH）之酵母菌株Saccharomyces cerevisiae GSHOL2以木薯澱粉為進料，依序以饋料－批次式發酵生產生質酒精及GSH，於發酵生產生質酒精46小時後與繼續發酵生產GSH 36小時後，分別可得12.0%（wt%）生質酒精及19.4 mg/g-DCW GSH。再利用上述木薯澱粉發酵酒精之殘渣廢液為進料基質，以Cl. acetobutylicum S1厭氧發酵生產生質丁醇，其最大丁醇產量達3.1 g/L，丁醇產率為0.40 mol butanol/molsugar。本整合性生物製程可生產生質酒精及生質丁醇作為生質燃料，並產出麩胱甘肽酵母菌粉為生技產品之主原料，可提升整體附加價值以降低總生產成本。

關鍵字

整合性生物製程 ； 生質酒精 ； 生質丁醇

並列摘要

A fusion yeast strain, ”Saccharomyces cerevisiae” GSHOL2, produced both of ethanol and glutathione (GSH) has been screened. The process optimization of fermentative productions of ethanol and GSH by ”Saccharomyces cerevisiae” GSHOL2 has been carried out using cassava starch as raw materials, 12.0% (wt%) ethanol and 19.4 mg/g-DCW GSH were obtained after GSH produced for 46 hrs following by ethanol produced for 36 hrs with fed-batch and fed-batch fermentation in sequence. In a jar fermenter, Cl. ”acetobutylicum” S1 utilized 23 g/L reducing sugar and produced 3.1g/L biobutanol, while the overall biobutanol yield was 0.40 mol butanol/mol reducing sugar. The integrated bioprocess discussed in this study could be used to produce bioethanol and biobutanol as biofuel. The glutathione-contained yeast powder could be used as raw biomaterials of functional food. The results can not only increase additional economic values, but also reduce the total cost of production. Furthermore, it can also be a reference for commercialization in the future.

並列關鍵字

Bioethanol ； Glutathione ； Biobutanol