澱粉酒精殘渣生產生質丁醇之生物製程開發

Bioprocess Development for Production of Biobutanol from the Starch Alcohol Residues

林忠亮(Chung-Liang Lin) ； 林士民(Shyh-Mim Lin) ； 湯雅理(Ya-Li Tang) ； 李旻倉(Min-Cang Li) ； 陳文章(Wen-Chang Chen) ； 王明妤(Ming-Yeu Wang)

《中華生質能源學會會誌》 31卷3&4期 (2012/12) Pp. 87-96

https://doi.org/10.6385/JBESC.201212.0087

摘要

本製程以提升整體附加價值及降低總生產成本為主要考量，先以酵母融合菌株利用木薯澱粉為料源發酵生產酒精與麩胱甘肽，再利用上述木薯澱粉酒精之殘渣廢液為進料基質，以Cl. Acetobutylicum S1厭氧發酵生產生質丁醇。以10公升發酵槽，不添加其他營養源，接種發酵培養72小時後，最大丁醇產量達3.41 g/L。添加10 mg/L NaN_3可以抑制雜菌汙染且提升丁醇產量達4.03 g/L;添加15 mM CH_3COONa 亦可提升丁醇產量至4.62g/L;若殘渣廢液中加入50 g/L糖蜜為料源，接種發酵培養84小時後將可大量提升Cl. Acetobutylicum S1之丁醇產量達11.71 g/L。以不織布為載體固定化Cl. acetobutylicum S1菌體生產丁醇結果與游離菌體相近。上述結果顯示該菌株具有工業化應用之潛力。

關鍵字

澱粉酒精殘渣 ； 生質丁醇 ； 固定化菌體

並列摘要

The purpose of this study is to optimum bioprocess for biobutanol production from the cassava starch alcohol residues, and to achieve economic benefits. Bioethanol and glutathione were produced using cassava starch for fusion yeast by sequentially fed-batch fermentation. The starch alcohol residues was reused to feed the CL acetobutylicum SI anaerobic fermentation, and 3.41 g/L of biobutanol was produced in 72 h-batch jar fermentation. The addition of NaN_3 and CH_3COONa in media could improve biobutanol production. In 10 L jar fermentator test, addition of 50 g/L cane molasses to the starch alcohol residues were used as substrate, Cl. acetobutylicum SI utilized it and produced 11.71 g/L biobutanol. The immobilized Cl. acetobutylicum SI have similar performance on biobutanol production. The results showed that immobilized Cl. acetobutylicum SI had the potential for industrial application of biobutanol.

並列關鍵字

Starch alcohol residues ； Biobutanol ； Immobilization cell