MODIFIED PHASE CONTRAST MICROSCOPY AND ITS APPLICATIONS TO THE OBSERVATION OF MARINE BIOLOGICAL OBJECTS

富氏顯微光學技術之改良及其應用于海洋生物體之觀測

施明昌(Ming Chang Shih) ； 黃將修(Jiang-Shiou Hwang)

《Journal of Marine Science and Technology》 8卷1期 (2000/06) Pp. 61-64

https://doi.org/10.6119/JMST.200006_8(1).0007

摘要

我們改良富氏光學配合近紅外線照射以達成高頻影像對比的效果以及展示觀察之微小生物體的微細構造，我們應用這個改良的光學系統去研究豐年蝦在三度空間之水體中的游泳行爲以及觀測活體海膽的刺及足之顯微結構及運動過程，本研究中的改良之影像系統乃是由平行的雷射光束、富氏光學轉變系統還有近紅外光源和CCD影像錄影系統組合而成。我們從得到的影像中可以追踪豐年蝦游泳的軌跡以及每一個螢幕的慢動作過程我們也可以觀察到海膽的刺和足受環境影響時的反應。

並列摘要

We demonstrate the imaging technique for the study of marine micro-biota(e.g.size ranged from um to cm)by modifying the Fourier optics with a near IR illuminating source to improve the contrast of the high spatial frequency images and to reveal the micro-structure of the observed biological object. We also present the application of this system to the study of swimming behavior of the Artemia in free space, and the detailed structure of the spines and podia of the sea urchin. The imaging system is consisted of a collimated laser beam, a high pass Fourier optics transform system, a near IR illumination source, and a CCD digital imaging recording system. From the images recorded, we are able to track the swimming trajectory and frame-by-framemotion of Artemia, and to observe how the spines and podia of the sea urchin react to the stimulation of the environment.

並列關鍵字

Artemia ； Phase contrast microscopy ； sea urchin ； behavior