Mosquito Larvicidal Activity of Broussonetia papyrifera Compound Marmesin by Blocking Protein AeSCP-2, Docking Strategies, and Combined Effect of Copepod Megacyclops formosanus Against Dengue Vector Aedes aegypti (Diptera: Culicidae)

Kandasamy Kalimuthu ； Chia-Hsiang Wang ； Shiu-Mei Liu ； Li-Chun Tseng ； Kadarkarai Murugan ； Jiang-Shiou Hwang

《Journal of Marine Science and Technology》 21卷特刊 (2013/12) Pp. 308-315

https://doi.org/10.6119/JMST-013-1223-8

並列摘要

Mosquito-borne diseases that have an economic impact create losses in commercial and labor outputs, particularly in countries with tropical and subtropical climates. The emergence of resistance to synthetic insecticides is a challenge to mosquito control. Cyclopoid copepods are important predators in many aquatic ecosystems and have been successfully used as biological agents to control mosquito larvae. For this study, we examined the larvicidal activity of the copepod Megacyclops formosanus in combination with the compound marmesin (which was purified from the methanol crude extract of the plant stem bark of Broussonetia papyrifera) against Aedes aegypti larvae. Their larvicidal activity and in silico docking analysis regarding the inhibition of the binding cholesterol sterol carrier protein-2 (AeSCP-2) against A. aegypti were evaluated. The significant larvicidal potential was recorded after the marmesin plant compound treatment against the dengue vector A. aegypti. Larval mortality was observed after 24 h of exposure. The LC50 and LC90 of marmesin against the first to fourth instar larvae and pupae were 0.104, 0.115, 0.137, 0.176, and 0.353 ppm, and 0.255, 0.270, 0.297, 0.365, and 0.643 ppm, respectively. This study showed that marmesin and copepods can be used effectively for mosquito larvae control programs. This is an ideal eco-friendly approach for controlling A. aegypti larvae.

並列關鍵字

Aedes aegypti ； Broussonetia papyrifera ； sterol carrier protein-2 ； marmesin ； larvicidal

參考文獻

Abbott, W. S., “A method of computing the effectiveness of insecticides,”Jornal of Economic Entomology, Vol. 18, pp. 267-269 (1925).

Amusan, A. A., Idowu, A. B., and Arowolo, F. S., “Comparative toxicity effect of bush tea leaves (Hyptis suaveolens) and orange peel (Citrus sinensis) oil extract on larvae of the yellow fever mosquito Aedes aegypti,” Tanzania Journal of Health Research Bulletin, Vol. 7, pp. 174-178 (2005).

Barani Kumar, R., Shanmugapriya, B., Thiyagesan, K., Raj Kumar, S., and Xavier, S. M., “A search for mosquito larvicidal compounds by blocking the sterol carrying protein, AeSCP-2, through computational screening and docking strategies,” Pharmacognosy Research, Vol. 2, pp. 247-53 (2010).

Bellows, T. S., “Restoring population balance through natural enemy introductions,” Biological Control, Vol. 21, pp. 199-205 (2001).

Blitzer, E. J., Vyazunova, I., and Lan, Q., “Functional analysis of AeSCP-2 using gene expression knockdown in the yellow fever mosquito, Aedes aegypti,” Insect Molecular Biology, Vol. 14, pp. 301-307 (2005).