血庫自製PRP的效益評估

目的：高濃度血小板血漿（PRP）商用萃取套組可有效抽取PRP，因成本高及廠牌不同，抽取PRP的濃度也有差異。本研究的目的是在無菌過程建立自製PRP，經冷凍催化PRP，檢測生長因子的濃度，作為自製PRP的效益評估。材料與方法：分別抽取3位健康成人（檢體-1、檢體-2、檢體-3）各36 mL靜脈血，在無菌操作台內注入於4管ACD抗凝試管及1管EDTA抗凝試管中。採用二次離心法製備PRP，其中離心前後分別檢測檢體-1的血小板濃度，作為離心轉速的測試。檢體-2與檢體-3以pH試紙測定pH值，以ELISA方法（Arigo biolaboratories）檢測EGF濃度。分別置於-70℃冷凍1小時及1.5小時，活化PRP再分別檢測EGF的濃度，計算冷凍活化前與冷凍活化後的EGF濃度之差異。再次檢測pH值。以Thio培養基作細菌培養。比較自製PRP與商用單一萃取套組的費用。結果：自製方法在36 mL全血可萃取6 mL PRP，並可取得2.25倍的PRP。前後兩次的pH值為7－8。細菌培養結果都為陰性。經-70℃冷凍活化PRP後，EGF的濃度分別提高3.5倍與3.8倍。每位成人的自製費用比商用套組減少92.2%。結論：建立標準化的PRP無菌製備流程，可取得高濃度的PRP，以冷凍活化可提高EGF的濃度，且費用較低，可提升臨床應用之效益。

關鍵字

PRP ； EGF ；冷凍活化

並列摘要

Objective: Platelet-rich plasma (PRP) preparation kits are commercially available and various; however, there are disadvantages of PRP commercial kits including high cost and difference in concentration of obtained PRP. The aim of this study was to prepare PRP in a sterile manner by frozen activation. The concentration of growth factors was examined for evaluating effectiveness of the obtained PRP. Methods and material: Venous blood samples (36 mL/person) were collected from 3 healthy adults (Sample1-3). In sterile cabinet, each individual's sample was dispensed into 4 ACD-containing glass tubes and 1 EDTA-containing glass tube. PRP is prepared by a process of second centrifugation. To determine optimal centrifugation condition, platelet concentration of sample 1 was used to compare pre-and post-centrifugated difference. To compare the difference of pre-frozen and post-frozen in pH and EGF concentration, sample 2 and 3 were frozen at -70℃ for 1 h and 1.5 h. pH strips and EGF ELISA kit (Arigo biolaboratores) are used to measure results. Samples were inoculated into thioglycolate broth and incubated for operational contamination. The cost was compared between laboratory-developed PRP preparation and commercial PRP kits. Results: With laboratory-developed PRP preparation, the volume of 6 mL PRP product can be obtained from each healthy individual's 36 mL whole blood and the concentration of PRP product is 2.25 times that of whole blood. Pre-and post-frozen pH were 7-8. Bacterial incubation is no growth. With freeze-activated process of samples at -70℃, the concentration of EGF is 3.5 and 3.8 times higher than original samples. The laboratory-developed PRP preparation is beneficial to each individual for saving approximately 92.2% of spending than commercial PRP kits. Conclusion: Establishment of standardized sterile PRP preparation is beneficial to obtain high-concentrated PRP product, enhance the concentration of EGF via frozen activation and be cost-effective. In addition, this laboratory-developed PRP preparation is highly beneficial to the clinical practice.

並列關鍵字

Platelet-rich Plasma (PRP) ； Epidermal growth factors (EGF) ； frozen activation

國際替代計量

血庫自製PRP的效益評估

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