水稻stunted lemma/palea 1突變對偶基因的功能探討

Functional Characterization of the Stunted Lemma/Palea 1 Mutant Allele in Rice

侯雅玲(Ya-Ling Hou)

《臺東區農業改良場研究彙報》 25輯 (2015/10) Pp. 43-55

摘要

Stunted lemma/palea 1（slp1）為水稻突變品系，其外表型呈現矮株、內外穎退化與不稔。前人研究的結果顯示slp1突變外表型可能是由於OsSPL16蛋白序列中第六個胺基酸發生錯義突變而造成。本研究顯示過量表現正常OsSPL16對偶基因於slp1突變植株中並無法使slp1突變外表型回復正常，然而將突變OsSPL16對偶基因導入正常水稻植株中會造成轉殖植株呈現slp1突變外表型。此結果證實OsSPL16的錯義突變對偶基因確實為slp1突變對偶基因，而此突變對偶基因也具有活性，能影響水稻株高及花穗發育。

關鍵字

水稻 ； 內穎 ； 外穎

並列摘要

The rice stunted lemma/palea 1 (slp1) mutant displays dwarf, shorten panicle length, seriously degenerated lemma/palea, and sterility. Previous study suggested that a missense mutation at the sixth amino acid of the OsSPL16 protein was likely responsible for the slp1 mutant phenotypes. The current study showed that overexpression of the wild-type OsSPL16 allele in the slp1/slp1 and Slp1/slp1 mutants were unable to convert the slp1 mutant phenotype to normal. However, introduction of the mutant OsSPL16 allele into a normal rice cultivar resulted in the slp1 mutant phenotypes displayed in the transgenic plants. These results concluded the missense mutation of OsSPL16 is the slp1 mutant allele and a neomorph allele which affects plant height and development of inflorescence and spikelet.

並列關鍵字

Oryza sativa ； Lemma ； Palea ； OsSPL16