水稻基因體的同義編碼使用之分析

Analysis of Synonymous Codon Usage in Rice Genome

黃家瑀(Chia-Yu Huang)

指導教授 ： 趙雅婷

元智大學/工程學院/生物科技與工程研究所/碩士(2009年)

摘要

同一基因體中的不同基因，或是不同的基因體之間，選擇同義編碼的形式會有所不同。對於原核生物和真核生物來說，影響編碼使用偏好的因素不盡相同。本研究分析水稻基因體的編碼使用偏好，並探討在兩種品種、三種不同組織當中，水稻基因之表現量和編碼使用偏好之間的關係。密碼子第三位置的GC含量與編碼偏好有高度相關。此外，具有高度編碼偏好的基因其表現量顯著高於低度編碼偏好的基因。本論文並進一步分析高度編碼偏好之基因功能並進行分類。

關鍵字

同義編碼 ； 水稻 ； 生物資訊

並列摘要

Patterns of synonymous codon usage vary among genes within a genome and different genomes as well. It is called codon usage bias when the amino acids show the preference for particular nucleotide at the third position of codons. In eukaryotes the codon usage bias is very complicated, and it is associated with many biological factors. This research analyzes the codon usage patterns in rice genome, as well as the relationship between the gene expression levels and the codon usage bias. The results show that there is significant correlation between the GC3 content and the measurements of codon usage bias. The higher the GC3, the stronger the codon usage bias is. Besides, higher-codon-bias genes expressed at significantly higher level than lower-codon-bias genes do. The biological functions and cell localizations of the high-codon-bias genes were assessed based on the Gene Ontology analysis.

並列關鍵字

synonymous codon ； rice ； bioinformatics

參考文獻

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