五味子素Schisandrin B輻射保護作用之探討

An Approach of Radioprotective Effects of Schisandrin B

王銘志(Ming-Chih Wang) ； 柯燿筆(Yaw-Bi Ker) ； 王乃弘(Nai-Phon Wang) ； 喬長誠(Charng-Cherng Chyau) ； 賴易成(Yih-Cherng Lai)

《放射治療與腫瘤學》 16卷3期 (2009/09) Pp. 203-213

https://doi.org/10.6316/TRO/200916(3)203

摘要

Purpose: Schisandrin B (San B), an active component of Schizandra chinensis Baill, has biological activities and pharmacological action including anti-oxidative and hepatoprotective effects. In this present study, we investigated the radioprotective effect of San B and its related mechanism.
Material and Methods: Cytotoxicity of pre-exposure to San B 72 hrs on Chang liver cell was measured by MTT assay. The radiation-induced apoptosis of Chang liver cell pretreated with San B at 40 μM for 24 hours followed by irradiation 15 Gy was assessed by flow cytometry and Western Blot test. The antioxidative activity of Chang liver cells pretreated with San B 40 μM for 4 hours followed by irradiation was evaluated by Glutathione Assay Kit.
Results: A significant increase in mean survival viability (31.4±13.6%) of Chang liver cell irradiated after pre-exposure to San B at 40 μM was observed as compared to corresponding irradiated alone controls (22.6±9.6%, p＜0.05) by MTT assay. The sub G1 population of Chang liver cells, pretreated with San B at 40 μM followed by irradiation, was 5% compared to 12% in radiation alone group (p＜0.05). The antioxidative activity of Chang liver cells pretreated with San B 40 μM followed by irradiation also significantly increased the intracellular glutathione (GSH) levels in comparison with radiation alone group. Furthermore, Western blotting showed that pretreatment with San B 40 μM significantly down-regulated p53 and Bax, suppressing expression of caspases-9 and caspases-3 after irradiation. However, no effect on the antiapoptotic protein Bcl-2 was found, suggesting that the radioprotective effect of San B is mediated by inhibition of p53-mediated apoptotic pathway.
Conclusion: Our results suggest that San B has a radioprotective effects on Chang liver cells. Its action mechanism involves both elevation of antioxidative activity and inhibition of radiation-induced apoptosis.

關鍵字

五味子素 ； 輻射保護 ； 抗凋亡 ； 抗氧化

並列摘要

Purpose: Schisandrin B (San B), an active component of Schizandra chinensis Baill, has biological activities and pharmacological action including anti-oxidative and hepatoprotective effects. In this present study, we investigated the radioprotective effect of San B and its related mechanism.
Material and Methods: Cytotoxicity of pre-exposure to San B 72 hrs on Chang liver cell was measured by MTT assay. The radiation-induced apoptosis of Chang liver cell pretreated with San B at 40 μM for 24 hours followed by irradiation 15 Gy was assessed by flow cytometry and Western Blot test. The antioxidative activity of Chang liver cells pretreated with San B 40 μM for 4 hours followed by irradiation was evaluated by Glutathione Assay Kit.
Results: A significant increase in mean survival viability (31.4±13.6%) of Chang liver cell irradiated after pre-exposure to San B at 40 μM was observed as compared to corresponding irradiated alone controls (22.6±9.6%, p＜0.05) by MTT assay. The sub G1 population of Chang liver cells, pretreated with San B at 40 μM followed by irradiation, was 5% compared to 12% in radiation alone group (p＜0.05). The antioxidative activity of Chang liver cells pretreated with San B 40 μM followed by irradiation also significantly increased the intracellular glutathione (GSH) levels in comparison with radiation alone group. Furthermore, Western blotting showed that pretreatment with San B 40 μM significantly down-regulated p53 and Bax, suppressing expression of caspases-9 and caspases-3 after irradiation. However, no effect on the antiapoptotic protein Bcl-2 was found, suggesting that the radioprotective effect of San B is mediated by inhibition of p53-mediated apoptotic pathway.
Conclusion: Our results suggest that San B has a radioprotective effects on Chang liver cells. Its action mechanism involves both elevation of antioxidative activity and inhibition of radiation-induced apoptosis.

並列關鍵字

Schisandrin B ； Radioprotection ； Antiapoptosis ； Antioxidation