間歇運動對蛋白質產生之影響

The Effects of Intermittent Exercise on Proteinuria

吳慧君(Huey-Chinn Wu) ； 江界山(chiang Jasson ph.D)

《體育學報》 13期 (1991/12) Pp. 129-145

https://doi.org/10.6222/pej.0013.199112.2011

摘要

本研究之目的有二：1.瞭解間歇運動後對健康青年男子血、尿中之總蛋白(TP)、白蛋白(ALB)及β2-微球蛋白(β2-mG)濃度及其排泄的影響；2.比較間歇運動前後尿蛋白SDS-PAGE及等電聚焦電泳圖譜之變化情形。
本研究是以浙江省杭州大學11名敎師及學生爲自願受試對象。運動開始前、後分別收集受試者之血尿樣本，再依其檢測項目，置於4℃及-20℃冰箱中保存，所有樣本均於當日檢測。所得資料以相依樣本t考驗統計方法處理，結果如下：
1.運動後BTP、UTP、BALB、UALB、Uβ2-mG、BLa、ULa及Bcr之濃度均顯著的高於運動前。
2.運動後UTP、UALB、Uβ2-mG及ULa之排泄率均顯著的高於運動前。
3.運動後UCr濃度及UCr排泄率顯著的低於運動前。
4.運動後ALB、β2-mG及La之血漿清除率均顯著高於運動前。
5.尿蛋白SDS-PAGE結果顯示，運動前尿液中蛋白質以ALB爲主；運動後呈高、低分子混合型蛋白尿。
6.由運動前後尿蛋白之IEP圖譜變化情形顯示，運動時尿液排泄的蛋白質主要是陰離子蛋白。

並列摘要

The purposes of this study were:1.To determine the affect of TP,ALB, β2-mG on level and execretion of young healthy men's blood and urine after intermittent exercise. 2. To compare the SDS-PAGE of proteinuria and Isoelectric focusing electrophoresis's difference pre and post interval exercise.
This research is based on results from 11 volunteer teachers and students of Han-Chou University Shr-Chang province who volunteered for the test.
The process consisted of collecting individual blood and urine samples pre and post exercise,then according to the item, storing at 4℃ and -20℃ respectively. All samples were analyzed on the day taken.
A paired T-test was utilized to analyze the data. On the basis of results from this study, it is concluded that:
1. The level of BTP, UTP, BALB, UALB, U β^2-mG,BLa,ULa,and BCr at post-exerciseare all significant higher than pre-exercise.
2. The execretion of UTP,BALB,Uβ2-mG,ULa is significant higher than pre-exercise.
3. The level and the execretion of UCr are significant lower than pre-exercise.
4. The plasma clearance rates of ALB,β^2-mG and La are identically higher than pre-exercise.
5. Proteinuria's SDS-PAGE result shows, that ALB is the main element in protein of urine at pre-exercise; after exercise it shows a mixed glomerular-tubular type.
6. Through the IEP of proteinuria pre-exercise and post-exercise pattern's changing situation shows, while at exercise the protine of urine execrete mainly is anion-protein.