耐力運動對人體骨骼肌抗氧化酶的影響

Antioxidant Enzyme Activities in Human Skeletal Muscle Response to Endurance Exercise

謝錦城(Chin-Cheng Hsieh)

《體育學報》 22期 (1997/01) Pp. 237-247

https://doi.org/10.6222/pej.0022.199701.3421

摘要

在先前多篇探討耐力運動對抗氧化酶的研究，大多採用老鼠的骨骼肌為研究對象，而沒有以人體的骨骼肌來進行研究。本篇的研究目的是以人類為受試對象，探討人體骨骼肌在騎腳踏車耐力運動的情況下，對於抗氧化酶的影響。受試者為六名男性與二名女性（年齡29.2±2.3），所從事的耐力運動為各人70%VO2max的運動強度，骑腳踏車一小時，在進行耐力運動的前後皆從受試者的股外側肌抽取骨骼肌。然後將抽取到的骨骼肌進行過氧化物歧化酶(SOD)、穀胱甘肽氧化酶(GPx)、穀胱甘肽還原酶(GRd)等抗氧化酶的生化分析。以重複量數t-test，考驗騎腳踏車耐力運動前後的人體骨骼肌抗氧化酶的差異情形。結果顯示，一小時的騎腳踏車耐力運動對於人體骨骼肌的過氧化物歧化酶、穀胱甘肽過氧化酶、穀胱甘肽還原酶等抗氧化酶皆沒有造成顯著性的差異。由於人體骨骼肌的抗氧化酶在中強度的騎腳踏車耐力運動下沒有受到影響，於是本研究認為中強度的耐力運動對於人體的骨骼肌可能不會造成氧化壓力(oxidative stress)。

關鍵字

耐力運動 ； 過氧化物歧化酶 ； 穀胱甘肱過氣化酶 ； 穀胱甘肱還原酶

並列摘要

Rat skeletal muscles were studied in most of previous studies concerning the response of endurance exercise on antioxidant enzyme activities, but not human skeletal muscles. The purpose of this study was to investigate the effect of cycling endurance exercise on antioxidant enzyme activities in human skeletal muscle. Muscle biopsies were taken from the vastus lateralis of 6 male and 2 female (29.2±2.3 yr.) before and after 1 hour cycling endurance exercise at 70% VO2max. The activities (U/mg protein) of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GRd) were measured. To determine whether there were significant differences on antioxidant enzyme activitiesin human skeletal muscle between before and after cycling endurance exercise, repeated t-test was run. The activities of SOD, GPx, and GRd all were not signify-cantly changed (p＞.05) resulting from cycling endurance exercise. These results indicate that moderate intensity cycling endurance exercise may not cause oxidative stress in human skeletal muscle due to unchanged antioxidant enzyme activities.

並列關鍵字

Endurance Exercise ； Superoxide Dismutase ； Glutathione Peroxidase ； Glutathione Reductase