單次離心運動對骨骼肌粒腺體釋出細胞色素C及細胞凋亡誘發因子的影響

The Effect of Single Bout Eccentric Exercise on Mitochondria Released Cytochrome C and Apoptosis Inducing Factor in Skeletal Muscle

陳聰毅(Tsung-I Chen) ； 林念璁(Nien-Tsung Lin)

《體育學報》 41卷4期 (2008/12) Pp. 15-28

https://doi.org/10.6222/pej.4104.200812.0802

摘要

Purpose: This study was to investigate the effect of eccentric exercise on mitochondria to release the cytochrome c (cyt-c) and apoptosis inducing factor (AIF) in skeletal muscle. Methods: Animals, male Sprague-Dawley rats, were randomly assigned to either a rest control group (N=4), an exercise control group (level running, LR; N=16) or an eccentric exercise group (downhill running, DHR; N=16). The rats in both LR and DHR ran on animal treadmill at a constant speed of 20 m/min on the level (0 degree incline) or downhill (a 16 degree decline) for 90 min. The 90 min running time was divided into 5 min runs separated by 2 min rest intervals (18 bouts). Rats were sacrificed at post-exercise 0 hour (PE0; n=4), post-exercise 24 hours (PE24; n=4), post-exercise 48 hours (PE48; n=4) and post-exercise 72 hours (PE72; n=4), respectively. The triceps brachii (TB) muscle was dissected out and frozen in liquid nitrogen rapidly. Results: The phosphorylated c-Jun NH2-terminal kinase (p-JNK) expression of DHR was significantly higher than LR at PE0 (p＜.05). The expression level of cytosolic cyt-c released from mitochondria of DHR which was significantly higher than LR at both PE24 and PE48 (p ＜ .05). The expression level of nuclei AIF of DHR was significantly higher than LR at PE24 (p ＜ .05). Conclusions: 1) A single bout eccentric exercise might increase the expression level of cytosolic cyt-c released from mitochondria in TB at both PE24 and PE48. 2) A single bout eccentric exercise didn't affect the expression level of cytosolic AIF released from mitochondria in TB. However, the expression level of nuclei AIF was increased at PE24 after a single bout eccentric exercise.

關鍵字

離心運動 ； 粒腺體 ； 細胞色素c ； 細胞凋亡誘發因子

並列摘要

Purpose: This study was to investigate the effect of eccentric exercise on mitochondria to release the cytochrome c (cyt-c) and apoptosis inducing factor (AIF) in skeletal muscle. Methods: Animals, male Sprague-Dawley rats, were randomly assigned to either a rest control group (N=4), an exercise control group (level running, LR; N=16) or an eccentric exercise group (downhill running, DHR; N=16). The rats in both LR and DHR ran on animal treadmill at a constant speed of 20 m/min on the level (0 degree incline) or downhill (a 16 degree decline) for 90 min. The 90 min running time was divided into 5 min runs separated by 2 min rest intervals (18 bouts). Rats were sacrificed at post-exercise 0 hour (PE0; n=4), post-exercise 24 hours (PE24; n=4), post-exercise 48 hours (PE48; n=4) and post-exercise 72 hours (PE72; n=4), respectively. The triceps brachii (TB) muscle was dissected out and frozen in liquid nitrogen rapidly. Results: The phosphorylated c-Jun NH2-terminal kinase (p-JNK) expression of DHR was significantly higher than LR at PE0 (p＜.05). The expression level of cytosolic cyt-c released from mitochondria of DHR which was significantly higher than LR at both PE24 and PE48 (p ＜ .05). The expression level of nuclei AIF of DHR was significantly higher than LR at PE24 (p ＜ .05). Conclusions: 1) A single bout eccentric exercise might increase the expression level of cytosolic cyt-c released from mitochondria in TB at both PE24 and PE48. 2) A single bout eccentric exercise didn't affect the expression level of cytosolic AIF released from mitochondria in TB. However, the expression level of nuclei AIF was increased at PE24 after a single bout eccentric exercise.

並列關鍵字

eccentric exercise ； mitochondria ； cytochrome c ； apoptosis inducing factor

參考文獻

Boppart, M. D.,Aronson, D.,Gibson, L.,Roubenoff, R.,Abad, L. W.,Bean, J.(1999).Eccentric exercise markedly increases c-Jun NH (2)-terminal kinase activity in human skeletal muscle.Journal of Applied Physiology.87(5),1668-1673.

Daugas, E.,Susin, S. A.,Zamzami, N.,Ferri, K. F.,Irinopoulou, T.,Larochette, N.(2000).Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis.The FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology.14(5),729-739.

Dirks, A. J.,Leeuwenburgh, C.(2002).Apoptosis in skeletal muscle with aging.American Journal of Physiology. Regulatory, Integrative and Comparative Physiology.282(2),519-527.

Kocturk, S.,Kayatekin, B. M.,Resmi, H.,Acikgoz, O.,Kaynak, C.,Ozer, E.(2008).The apoptotic response to strenuous exercise of the gastrocnemius and solues muscle fibers in rats.European Journal Applied Physiology.102(5),515-524.

Lim, J. H.,Kim, D. Y.,Bang, M. S.(2004).Effects of exercise and steroid on skeletal muscle apoptosis in the mdx mouse.Muscle and Nerve.30(4),456-462.