CYP2E1與GST基因多型性對二甲基甲醯胺職業暴露之生物指標之影響

Effects of Genetic Polymorphism of CYP2E1 and GST on Urinary Biomarkers of Occupational Exposure to N,N-dimthylformamide

吳俊德(Jyun-De Wu) ； 邱幼青(Yu-Ching Chiu) ； 林鍚慧(Hsi-Hui Lin) ； 戴火木(Huo-Mu Tai) ； 石東生(Tung-Sheng Shih) ； 張火炎(Ho-Yuan Chang)

《勞工安全衛生研究季刊》 14卷4期 (2006/12) Pp. 253-263

https://doi.org/10.7005/JOSH.200612.0253

摘要

本研究目的是探討職業暴露二甲基甲醯胺(N,N-dimethylformamide, DMF)之作業勞工體內CYP2E1及GST不同基因型(genotypes)對DMF尿液中DMF、MFF及AMCC三種生物指標物的影響。本研究選取某合成皮製造廠49位男性作業員工，進行全程勞工個人DMF暴露測定，並收集勞工下班後的尿液進行尿中DMF、NMF及AMCC的分析，及進行勞工體內CYP2E1(Pst1浏览及Dra1)及GST(TI/M1)基因多型性的分析。空氣DMF暴露濃度(ppm)及尿中DMF、NMF及AMCC濃度(mg/L)平均值為7.59、0.76、9.36及15.29。本研究發現CYP2E1之基因型和三種DMF尿中指標物皆未呈現顯著相關，而尿中AMCC濃度除受到空氣中DMF暴露值影響外，尚受到受測者體內GSTTI及GSTMI之基因型分布之影響，建議以尿中AMCC作為DMF職業暴露之內在指標時，應考量GST之基因多型性，方不致對DMF暴露產生錯估。

關鍵字

二甲基甲醯胺 ； 生物偵測 ； 基因多型性

並列摘要

The purpose of this study is to investigate the effects of genetic polymorphism of CYP2E1 and GST on three urinary biomarkers of N, N-dimethylformamide (DMF) exposure. Forty-nine male workers in a synthetic leather factory were environmentally monitored on their full work-shift exposure to DMF. DMF, N-methylformamide (NMF) and N-acetyl-S-(N-methylcarbamoy1) cysteine (AMCC) in urine were collected and analyzed immediately after the shift change. Their genetic polymorphism of CYP2E1 (Pst1 and Dra1), and GST (T1 and Ml) was determined by PCR (Polymerase Chain Reaction) and RFLP (Restriction fragment length polymorphism) from the peripheral lymphocytes. It was found the average concentrations of DMF in the air and DMF, NMF, and AMCC in urine were 7.59 ppm, 0.76, 9.36, and 15.29 mg L, respectively. No biomarkers in urine presented association with the CYP2E1 variants. Urinary AMCC, however, revealed significant association with the variants of both GSTT1 and GSTM1 after adjustment for DMF concentrations in the air. It is suggested that the use of urinary AMCC as a biomarker of DMF exposure should take the GST variants into account to avoid erroneous exposure estimates.

並列關鍵字

N,N-dimethylformamide DMF ； biological monitoring ； genetic polymorphism

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