Effects of Acetaminophen, Etodolac and Indomethacin on Migration, Proliferation and Collagen Expression of Tendon Cells

非類固醇抗炎藥物對肌腱纖維細胞移行、增生及膠原蛋白合成的影響

楊雲明(Yun-Ming Yang) ； 張翔寧(Hsiang-Ning Chang) ； 蘇中慧(S Jong-Hwei Pang) ； 侯鐘堡(Chung-Bao Hou) ； 蔡文鐘(Wen-Chung Tsai)

《台灣復健醫學雜誌》 42卷3期 (2014/09) Pp. 161-169

https://doi.org/10.6315/2014.42(3)04

摘要

背景：非類固醇抗炎藥物（NSAID）常被使用在肌腱發炎的情況。此類藥物因可抑制環氧合酶（COX）的活性，可以減輕患部的腫脹疼痛，並可促進及早活動。但有先前研究顯示，非類固醇抗炎藥物（NSAID）如celebrex，ibuprofen會抑制肌腱纖維細胞的移行，增生及合成膠原蛋白，進而有礙於患處的復原。目的：本實驗選擇檢測非典型NSAID（acetaminophen）及非選擇性長效NSAID（etodolac、indomethacin）對肌腱纖維細胞移行、增生及膠原蛋白合成的影響。方法：大鼠肌腱細胞經加入藥物培養後，運用細胞遷移實驗（transwell filter migration assay）以及細胞存活率分析（MTT assay）來檢測纖維母細胞的移行及增生。並使用即時定量聚合酶連鎖反應（real-time polymerase chain reaction）來檢測第一型及第三型膠原蛋白之合成。結果：細胞遷移實驗中，etodolac組和indomethacin組纖維母細胞之移行個數明顯降低，此抑制效果具統計學上意義；Acetaminophen組纖維母細胞移行個數與對照組相較無變化。細胞存活率分析和即時定量聚合酶連鎖反應中，Acetaminophen組、etodolac組和indomethacin組纖維母細胞增生率和第一型或第三型膠原蛋白合成，與對照組相較無變化。結論：Acetaminophen對於肌腱纖維母細胞的移行、增生及膠原蛋白合成無影響。而etodolac和indomethacin則與其他非選擇性非類固醇抗炎藥物相同，對於細胞的遷移有抑制效果，而對細胞增生或膠原蛋白合成則無影響。

關鍵字

對乙醯氨基酚 ； 依托度酸 ； 吲哚美辛 ； 非類固醇抗炎藥物 ； 肌腱癒合

並列摘要

Background: Non-steroidal anti-inflammatory drugs (NSAIDs) are commonly used to treat tendinopathy. Tendon healing requires migration of tendon cells to the repair site, followed by proliferation of these cells and collagen synthesis. Purpose: This study evaluated the effects of acetaminophen, etodolac, and indomethacin on tenocyte migration, proliferation, and synthesis of type I and III collagen. Methods: Rat Achilles tendon cells were treated with acetaminophen, etodolac, and indomethacin. Tendon cell migration and proliferation were assessed using transwell filter migration assay and MTT assay, respectively. The expression of collagen I and III mRNA were evaluated by quantitative real-time polymerase chain reaction. Results: Unlike acetaminophen, etodolac and indomethacin significantly reduced tenocyte migration (p ＜ 0.05). No effect of acetaminophen, etodolac, and indomethacin on cell proliferation was observed. The gene expressions of collagen I and III were not affected by acetaminophen, etodolac, or indomethacin treatment. Conclusion: Acetaminophen has no effect on tendon cell migration, proliferation, and type I and III collagen synthesis. Etodolac and indomethacin, similar to other non-selective NSAIDs, inhibit tendon cell migration but have no effect on tendon cell proliferation and type I and III collagen synthesis.

並列關鍵字

acetaminophen ； etodolac ； indomethacin ； non-steroidal anti-inflammatory drugs ； tendon healing ； Acetaminophen ； Etodolac ； Indomethacin