Effects of Lidocaine on Migration, Proliferation, Collagen Expression and Cytotoxicity of Tendon Cells

Lidocaine對肌腱細胞移行，增生，膠原蛋白表現量之影響及毒性之研究

張翔寧(Hsiang-Ning Chang) ； 蘇中慧(Jong-Hwei S Pang) ； 林妙穗(Miao-Sui Lin) ； 蔡文鐘(Wen-Chung Tsai)

《台灣復健醫學雜誌》 46卷2期 (2018/12) Pp. 71-79

https://doi.org/10.6315/TJPMR.201812_46(2).0003

摘要

Background: Lidocaine is commonly used for the treatment of a variety of tendinopathies in combination with corticosteroids injection. Purpose: The goal of this study was to evaluate the effect of lidocaine on migration, proliferation, protein expressions of type I and III collagen and cytotoxicity of tendon cells. Methods: Rat Achilles tendon cells were divided into control and study groups (treated with 0.1%, 0.15%, and 0.2% lidocaine). Tendon cell migration and proliferation were assessed using transwell filter migration assay and MTT assay, respectively. Cytotoxicity of lidocaine was evaluated by the LDH-Cytotoxicity assay. The protein expression of type I and type III collagen were evaluated by immunocytochemical staining. Results: Lidocaine significantly reduced tenodon cell migration (p ＜ 0.001). Cell death was measured by LDH assay and cytotoxicity increased dose-dependently (p ＜ 0.001). A dose-dependent lidocaine inhibition on tendon cell proliferation was demonstrated by MTT assay (p＜0.001). Immunocytochemical staining revealed that the protein expression of type I and III collagen were not affected by lidocaine treatment Conclusion: Lidocaine inhibits tendon cell migration and proliferation. Lidocaine induced cell death on tendon cells. However, the protein expression of types I and III collagen remained unchanged.

關鍵字

lidocaine ； local anesthetics ； tendons ； tendon healing

並列摘要

局部麻醉劑lidocaine常和類固醇（如dexamethasone）合併使用，作為注射藥物，用來治療多種肌腱相關疾病。本研究目的為探討lidocaine對肌腱細胞移行、增生和第一型及第三型膠原蛋白表現量之作用，以及可能對肌腱細胞存在之毒性。本研究方法為以不同濃度之lidocaine（0.1%, 0.15%, 0.2%）處理從Sprague-Dawley大鼠培養出的肌腱細胞，與控制組比較進行後續實驗。利用transwell filter migration assay檢測肌腱細胞移行能力。以MTT試驗檢測肌腱細胞增生。使用LDH-cytotoxicity assay分析lidocaine對肌腱細胞潛在之毒性。以免疫組織化學染色法（immunocytochemical staining）觀測lidocaine對第一型及第三型膠原蛋白表現量之影響。本研究結果顯示lidocaine顯著降低肌腱細胞移行能力（p＜0.001）。Lidocaine會加速肌腱細胞死亡且對肌腱細胞產生之毒性與lidocaine濃度呈現正相關（p＜0.001）。MTT試驗發現lidocaine會抑制肌腱細胞增生，lidocaine濃度越高抑制肌腱細胞增生越顯著（p＜0.001）。免疫組織化學染色法呈現第一型及第三型膠原蛋白表現量與有無接觸lidocaine並無顯著差別。本研究結論為lidocaine會抑制肌腱細胞移行及增生能力。Lidocaine會加速肌腱細胞死亡，但對第一型及第三型膠原蛋白表現量無顯著影響。

並列關鍵字

利多卡因（lidocaine） ； 局部麻醉劑（local anesthetics） ； 肌腱細胞（tendons） ； 肌腱修復（tendon healing）

參考文獻

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