In this study, Hicool, Coolmax, Coolplus first three speed dry wicking fabric capacity, diffusion rate, wicking test, production test data based bicycle clothing, with heart rate variability analysis, infrared thermal imager did not wear any fabric measuring, General wear cotton fabric, wicking fabric of three 10-minute static and dynamic data to design the best 30 minutes of cycling clothing, in response to the arms, chest, abdomen measuring infrared thermal imaging, heart rate variability analyzer comfort. The results showed clearly in the three quick-drying wicking fabric capacity, diffusion area, wicking test was significantly higher than normal cotton. Against the body under the arms, chest, abdomen design the best bike clothing heat conditions. Test results show that the fabric has a relatively good wicking comfort.