台灣肖楠開花旺盛與稀疏營養系激勃素轉型及劑量變化之偵測與鑑定

Identification and Quantification of Gibberellin Transformation and Dosage Changes in Good and Poor Flowering Clones of Calocedrus formosana Grafts

鍾振德(Jeng-Der Chung) ； 郭幸榮(Shing-Rong Kuo) ； 簡慶德(Ching-Te Chien)

《台灣林業科學》 18卷4期 (2003/12) Pp. 387-400

https://doi.org/10.7075/TJFS.200312.0387

摘要

本研究旨在探討大雪山台灣肖楠種子園開花旺盛營養系(no. 11)與開花稀疏營養系(no 22)經激勃素A3(GA3)處理後之開花效果，並定量分析在不同時期採收之芽體內各種不同激勃素的含量變化。以未經GA3處理之上述二個營養系和六龜種子園11號營養系作為對照組。經GA3處理之no. 11營養系開花比率99.7%，no. 22營養系85.6%，對照組六龜11號營養系7.1%，另二個未經處理者沒有花芽分化。激勃素定量分析在1999年5月下旬GA3處理之後，以間隔l及3 wk切取芽體頂端l-2 mm冷凍乾燥，秤取0.5g乾重萃取分析激勃素含量的變化。結果GA3處理之樣品測到大量GA1與GA3及微量的GA20，1wk後no. 11營養系GA1為920 ng•g^(-1)dry weight (dw)與GA3為l,200 ng•g^(-1)dw，no. 22營養系GA1為l,100 ng•g^(-1)dw與GA3為l,312 ng•g^(-1)dw。三個星期後no. 11營養系GA1為436 ng•g^(-1)dw與GA3為600 ng•g^(-1)dw，no. 22營養系GA1為l,118 ng•g^(-1)dw與GA3為826 ng•g^(-1)dw。兩個時間點的GA20含量過低無法定量。由GA1和GA3含量的變化，顯示no. 11營養系l和3 wk間濃度下降較大，而no. 22營養系濃度變化較小。由於芽體發現有GA24存在，證實台灣肖楠激勃素的合成路徑走non-13-hydroxylation pathway途徑，而激勃素的代謝速率影響花芽的分化。

關鍵字

台灣肖楠 ； 開花旺盛與稀疏營養系 ； 激勃素A3 ； 矮生稻生物檢測 ； 質譜儀

並列摘要

The flowering responses of GA3-treated branches of good flowering clone no. 11 and poor flowering clone no. 22 of Calocedrus formosana grown at Tashueshan were investigated. Quantitative analysis of gibberellins in buds harvested at different developmental stages was conducted as well. Samples collected from GA3-untreated clones grown at Tashueshan and clone no. 11 grown at Liukuei were used as controls. Flowering percentages of clone nos. 11 and 22 treated with GA3 were 99.7% and 85.6%, respectively. No flowers were found in the control clones of nos. 11 and 22, except for clone no. 11 grown at Liukuei which had a 7.1% flowering rate. Buds were collected at the 1 (superscript st) and 3 (superscript rd) weeks after branches were treated with GA3 in late May 1999. The tips of buds 1-2 mm long were excised and freeze-dried. Gibberellins extracted from 0.5 g of dry bud tips were quantified. Large amounts of GA1 and GA3 and minimal GA20 were detected in GA3-treated samples. The amount of GA1 and GA3 derived from clone no. 11 samples collected at the 1 (superscript st) and 3 (superscript rd) weeks were 920 and 1,200 ng•g^(-1) dry weight (dw), and 436 and 600 ng•g^(-1)dw respectively, while those from clone no. 22 were 1,100 and 1,312 ng·g^(-1)dw and 1,118 and 826 ng•g^(-1)dw respectively. The amounts of the decrease in GA1 and GA3 from the 1 (superscript st) to the 3 (superscript rd) week were greater in clone no. 11 than in clone no. 22. We failed to detect a minimal amount of GA20. Minimal GA24 was detected, suggesting that the biosynthesis of gibberellins might proceed through a non-13-hydroxylation pathway.

並列關鍵字

Calocedrus formosana ； good and poor flowering clones ； GA3 ； dwarf rice micro-drop bioassay ； GC-MS