喜樹之微體繁殖與喜樹鹼含量分析

Micropropagation and Evaluation of the Camptothecin Content of Camptotheca acuminata

張淑華(Shu-Hwa Chang) ； 蔡錦瑩(Jin-Yin Tsai) ； 許原瑞(Yen-Ray Hsui) ； 何政坤(Cheng-Kuen Ho)

《台灣林業科學》 20卷4期 (2005/12) Pp. 331-340

https://doi.org/10.7075/TJFS.200512.0331

摘要

本試驗以喜樹無菌種子苗之頂芽與含節莖段作為培植體，建立微體繁殖技術並分析其喜樹鹼含量。結果以頂芽比莖段適合作為微體繁殖的試驗材料，頂芽多芽體誘導最佳培養基為WPM添加0.01mg L^(-1) NAA與0.5mg L^(-1) BA，培養6wk後，每個頂芽平均可產生6.4個芽體。多芽體在WPM添加低濃度BA(0.1 mg L^(-1))培養基抽長後，將單一莖芽切下培養於WPM添加NAA或IBA培養基，以低濃度IBA(1mg L^(-1))處理最佳，可使莖芽100%發根且平均產生5.6根，組培苗出栽培養於苗圃1mo，苗木成活率可達95%以上。分析來自台北植物園、蓮華池、與中國江蘇省喜樹種子苗葉片的喜樹鹼含量，結果台灣種源的種子苗喜樹鹼含量顯著高於來自中國種源的種子苗，各種源種子苗間喜樹鹼差異很大，且種子苗與其組培苗間喜樹鹼的含量呈極顯著正相關，顯示利用微體繁殖大量培育含有高含量喜樹鹼的營養系苗，頗為可行。

關鍵字

喜樹 ； 微體繁殖 ； 喜樹鹼

並列摘要

Micropropagation and comptothecin content of shoot tips and nodal stem explants of Camptotheca aucminata, a plant known to have anti-cancer properties, derived from in vitro seedlings were investigated. Induction of multiple shoots from shoot tip explants was much greater than that from stem explants. The greatest shoot production induced from shoot tip explants was achieved on woody plant medium (WPM) with 0.01 mg L^(-1) α-naphthileneacetic acid (NAA)+0.5 mg L^(-1) benzyladenine (BA), forming an average of 6.4 buds per explant in 6 wk. Elongation of these multiple shoots was successful at a lower BA level (0.1 mg L^(-1)) on WPM. Individual shoots were rooted on WPM supplemented with indole-3-butyric acid (IBA) or NAA. A concentration of 1 mg L^(-1) IBA gave the highest percentage of rooting (100%) and root number (5.6 roots/shoot). The camptothecin content in leaves of seedlings and their micropropagated plants raised from the mother plants grown at Taipei Batonical Garden, and Lienhuachin Central, Taiwan were significantly greater than those from Jiangsu Province, China. Comptothecin of seedlings among the 3 provenances significantly differed. Since a highly positive correlation was found between the comptothecin content of seedlings and their micropropagated plants, this suggests that it is feasible to mass propogate C. acuminata plants cultured in vitro in order to obtain desired clones with high camptothecin content.

並列關鍵字

Camptotheca acuminate ； micropropagation ； camptothecin