Plant Regeneration through Somatic Embryogenesis from Young Leaves of Cinnamomum kanehirae Hayata

牛樟幼葉培養體胚發生與植株再生

陳盈君(Ying-Chun Chen) ； 張正(Chen Chang)

《台灣林業科學》 24卷2期 (2009/06) Pp. 117-125

https://doi.org/10.7075/TJFS.200906.0117

摘要

從牛樟幼葉誘導生成黃白色、硬實粒狀的胚性癒傷組織，穩定地繼代培養於含1 mg L^(-1) BA及0.5mg L^(-1) NAA之基礎培養基(CI medium)。牛樟胚性癒傷組織移入未添加生長調節劑之WPM培養基並置於5℃環境下培養14天，再移到25℃的環境下培養6週，可於癒傷組織表面觀察到體胚形成。牛樟各種型態之體胚同時移入含0.2 mg L^(-1) GA3 and及150 ml L^(-1)椰子水的WPM培養基2個月後，再移入不含生長調節劑之WPM培養基10個月，每2個月繼代一次，有31.4%體胚發芽長出胚根及胚芽而再生成小植株，其中僅正常型態體胚能順利發芽，畸型體胚則逐漸褐化。株高5公分的再生株經馴化及移植後具有83%(33/40)的存活比率，再生株於國立自然科學博物館植物園展覽區栽培3年，胸徑達6 cm，株高4 m。

關鍵字

體胚 ； 牛樟 ； 再生 ； 癒傷組織

並列摘要

Embryogenic calli of Cinnamomum kanehirae Hayata, are yellowish-white, compact, and granular, which were induced from young leaves, and were maintained on half-strength macroand micro-elements of MS media containing 1 mg L^(-1) 6-Benzyl aminopurine (BA) plus 0.5 mg L^(-1) 1-naphthalene acetic acid (NAA). Embryogenic calli were cultured at 5℃ for 14 d on woody plant medium (WPM) with no plant growth regulators, and then somatic embryos appeared on the surface of these calli after they were transferred to 25℃ for another 6 wk. Of somatic embryos, 31.4% germinated that were cultured on WPM supplemented with 0.2 mg L^(-1) GA3 and 150 ml L^(-1) coconut milk in 2 mo and then transferred to medium without plant growth regulators for another 10 mo with 2-mo intervals of subculturing. Regenerating plantlets of 5 cm in height were acclimated and transplanted to a greenhouse with an 83% survival rate. Subsequently, these plantlets were transferred to the Botanic Garden of the National Museum of Natural Science for 3 yr, after which the diameter at breast height was 6 cm, and the height was 4 m.

並列關鍵字

somatic embryo ； Cinnamomum kanehirae Hayata ； regeneration ； callus