精胺酸－甘胺酸－天門冬胺酸胺基酸序列於牙周組織工程之應用

Application of Arginine-Glycine-Aspartic Acid (RGD) Peptides in Periodontal Tissue Engineering

李湘晴(Chin-Chih Lee) ； 林喬棣(Chiao-Dih Lin) ； 金玉堂(Yu-Tang Chin) ； 江正陽(Cheng-Yang Chiang) ； 沈一慶(E-Chin Shen) ； 邱賢忠(Hsien-Chung Chiu) ； 傳鍔(Earl Fu)

《中華民國牙周病醫學會雜誌》 11卷1期 (2006/03) Pp. 31-43

摘要

牙周再生，首重於細胞附著。而精胺酸－甘胺酸－天門冬胺酸胺基酸序列(Arginine-Glycine-Aspartic acid peptides, Arg-Gly-Asp, RGD)為細胞附著關鍵因子之一。RGD胺基酸序列可分為兩類。一種為線狀的胺基酸序列，可直接影響接受器的親合性、接受器的選擇性及其它的生物特質。另一種為環狀胺基酸序列，是由其他胺基酸組合成環狀的系統，更能抵抗蛋白質水解，所以比線狀RGD更具應用性。RGD胺基酸序列可促進纖維母細胞及造骨細胞附著及促進細胞的展開和局部接觸的形成。在材料表面接枝附著性的縮氨酸(peptides)，是細胞及組織工程中所熱衷的發展區域。在生物材料上覆蓋RGD縮氨酸，可作為細胞與材料間介面之控制及達到生物活性促使人工植入物的終極目標。本文獻回顧將探討RGD為細胞附著之一關鍵因子、RGD與整合素(integrin)之結合、RGD的分類、RGD對纖維母細胞及造骨細胞附著的影響及應用於臨床醫學工作的發展等，寄望對牙周醫學作更深入探索。

關鍵字

精胺酸－甘胺酸－天門冬胺酸胺基酸序列 ； 附著 ； 牙周組織工程

並列摘要

The first step in periodontal regeneration is cell attachment. Arginine-Glycine-Aspartic acid (RGD) peptide is a key molecule for cell adhesion. Two types of RGD peptides have been classified. Linear RGD peptides could affect receptor affinity, selectivity, and other biologic properties. Cyclic RGD peptides sequence is flanked by other amino acids to build a ring system. Cyclic RGD peptides are much more potent than linear RGD peptides due to their resistance against proteolysis. RGD peptides enhance fibroblast and osteoblast attachment, flattening, and focal contact formation. Peptides-modified-surface is a hot and important developmental area in cell and tissue engineering. Biomaterials surface coated with RGD could control cell to material and biology implant in fantasy strategy. The purpose of this review paper is to discuss the RGD peptides (the key factor in cell attachment), the bind of RGD to integrins, the classification of RGD peptides and the enhancing ability of RGD on the cellular attachment of fibroblast and osteoblast, and the application of RGD peptides in periodontal tissue engineering.

並列關鍵字

RGD peptides ； cell attachment ； periodontal tissue engineering