高強度低彈性模數牙科用Ti-5Cr-9Mo合金表面改質之研究

本研究選用c.p. Ti和Ti-5Cr-9Mo 合金為基材，首先利用陽極氧化方法合金表面形成奈米管柱，接著在利用 RGD 胜肽於表面進行接枝，希望提升生物相容性。研究結果顯示c.p.Ti和Ti-5Cr-9Mo合金於0.3 M NaCl和 0.14 M NH4F 混合電解液中，以適當定電壓可形成均勻奈米管陣列，再經由 450℃ 持溫4小時熱處理，可得到銳鈦礦相之結晶結構，其中c.p. Ti之奈米管內徑為40~50 nm和管長為 300 nm。Ti-5Cr-9Mo合金之奈米管內徑為40~50 nm和管長為 250 nm。在人工模擬體液之耐蝕性方面，表面具有二氧化鈦奈米管陣列的c.p. Ti 與 Ti-5Cr-9Mo合金經過450℃熱處理後，腐蝕電位(Ecorr)會增加和腐蝕電流密度(Icorr)會降低，表示合金表面製作奈米管陣列，經過適當熱處理，可以提高耐蝕性。XPS 分析之結果顯示c.p. Ti 經陽極氧處理後所形成的奈米管陣列，均由二氧化鈦化合物所組成，鈦是以Ti4+ 形式存在。Ti-5Cr-9Mo 合金所形成的奈米管陣列，一部分由二氧化鈦化合物所組成，另一部分分別由鉻和鉬的氧化物所組成。二氧化鈦奈米管陣列中之鈦是以Ti4+ 形式存在，而鉻與鉬分別是以 Cr3+ 與 Mo4+ 存在。FTIR分析證實c.p. Ti 與 Ti-5Cr-9Mo合金表面經陽極氧化處理後，再利用浸泡共價鍵結合的方式可以成功接枝 OMP胜肽。生物相容性結果證實有接枝 OMP 胜肽的 c.p. Ti 與 Ti-5Cr-9Mo合金表現出最佳的細胞生長活性。

關鍵字

生物相容性；胜肽；耐蝕性；鈦合金；奈米管

並列摘要

In this study, cp Ti and Ti-5Cr-9Mo alloy were used as the substrates. In order to enhance biocompatibility of the substrates, the nanotubes were fabricated by anodic oxidation and the RGD peptides were grafted on the surface. The corrosion resistance of the substrates with a nanotube surface was examined. The results show that the highly ordered nanotube oxide layers developed on the surface of c.p. Ti and Ti-5Cr-9Mo alloy in the 0.3 M NaCl and 0.14 M NH4F mixed solution at an appropriate electric potential for one hour. After being annealed at 450℃ for four hours, the nano-tubes transformed into a rutile crystal structure. The average inner diameter of the nanotubes formed on c.p. Ti was 40 ~ 50 nm, with a tube length of 300 nm. The average inner diameter of the nanotubes formed on Ti-5Cr-9Mo alloy was 40 ~ 50 nm, with a tube length of 250 nm. When testing the corrosion resistance of the nanotubed surface of c.p. Ti and Ti-5Cr-9Mo alloy in Hank''s solution after heat treatment, the corrosion potential (Ecorr) increased and the corrosion current density (Icorr) reduced when compared with untreated specimens. This result shows that specimens with nanotubes can still maintain good corrosion resistance. XPS analysis results showed that the nanotubes on c.p. Ti are composed of TiO2 and on Ti-5Cr-9Mo alloy are composed of TiO2, Cr2O3 and MoO2. FTIR analysis confirmed that the surface of the nanotubed c.p. Ti and Ti-5Cr-9Mo alloy can be successfully grafted with OMP peptide by covalent bonding. Biocompatibility results showed that grafting OMP peptide to nanotubed cp Ti and Ti-5Cr-9Mo alloy produced the best cell growth activity.

並列關鍵字

biocompatibility ； corrosion resistance ； titanium alloy ； nano-tube ； peptide

參考文獻

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高強度低彈性模數牙科用Ti-5Cr-9Mo合金表面改質之研究

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