The purpose of this research was to determine the genetic knowledge, attitude, and needs for genetic education among clinical nurses. A cross-sectional study was conducted via self-structured questionnaires. Five hundred and eighty-three questionnaires were returned (73.33%). Significant results were as follows: the genetic knowledge among clinical nurses was poor, the genetic attitude of the clinical nurses was positive; the clinical nurses' needs for on-the-job training were high. There was positive association among genetic knowledge, attitude and educational needs. The result of this study helps to understand the clinical nurses' needs for on-the-job training. It can serve as reference for genetics educational program in clinical nurses.