中藥黃芩、槐花與薑黃抗發炎效果之比較

Comparisons of the Anti-inflammatory Effect between Scutellaria baicalensis、Sophora japonica and Curcuma longa

謝明學(Ming-Shueh Shieh) ； 周瑞玲(Jui-Ling Chou)

《元培學報》 15期 (2008/12) Pp. 37-45

https://doi.org/10.30171/JYUST.200812.0004

摘要

活化巨噬細胞大量生成一氧化氮(NO)的現象在發炎反應的許多病理進展過程中，扮演一個重要的指標。本研究以對lipopolysaccharide (LPS)刺激活化之RAW 264.7細胞的NO生成抑制試驗法，探討黃芩、槐花與薑黃50%乙醇粗抽物抗發炎作用。結果顯示，黃芩、槐花與薑黃50%乙醇粗抽物都具有抗發炎效果，其抑制NO生成的IC50各爲0.20mg/mL、2.35mg/mL及0.64mg/mL。同時，在添加相同濃度(0.4mg/mL)下，以未添加待測藥物NO生成量爲100%時，其生成 NO 量各自爲18±1.1%（黃芩）、87±1.6%（槐花）和71.6±2.9%（薑黃）。經比較結果，三種中藥50%乙醇粗抽物中，黃芩抗發炎的能力最強，而薑黃略優於槐花。

關鍵字

抗發炎 ； 巨噬細胞 ； 黃芩 ； 槐花 ； 薑黃 ； 一氧化氮

並列摘要

Overproduction of nitric oxide (NO) by activated macrophage play a central role in many pathological processes during inflammation. In this study, the anti-inflammatory effect of the Chinese herb Scutellaria baicalensis, Sophora japonica and Curcuma longa were assessed by measuring the decreased amounts of NO production by lipopolysaccharide (LPS) activated murin macrophage-like RAW 264.7 cells compared with that of drug-untreated control sample. The results showed that the 50% ethanol crude extracts of Scutellaria baicalensis, Sophora japonica and Curcuma longa suppressed NO production with the values of 50% inhibitory concentration (IC50) about 0.20, 2.35 and 0.64 mg/mL, respectively. Meanwhile, by addition of same concentration (0.4mg/mL) of these tested crude herb extracts, Scutellaria baicalensis, Sophora japonica and Curcuma longa produced 18±1.1%, 87±1.6% and 71.6±2.9% of NO production, when the NO produced by drug-untreated control samples was treated as 100%. The results indicated that anti- inflammatory effect of these three 50% ethanol crude extracts, Scutellaria baicalensis exerted the strongest potent, and Curcuma longa was better than Sophora japonica.

並列關鍵字

Anti-inflammation ； Macrophage ； Scutellaria baicalensis ； Sophora japonica ； Curcuma longa ； Nitric oxide