中藥黃芩及其活性成分之抗發炎作用研究

Study on the Anti-inflammatory Effect of Scutellaria Baicalensis Georgi and Its Active Constituent

謝明學(Ming-Shueh Shieh) ； 周瑞玲(Jui-Ling Chou)

《元培學報》 19期 (2012/12) Pp. 23-32

https://doi.org/10.30171/JYUST.201212.0003

摘要

巨噬細胞在生物體內扮演著調控先天免疫及發炎作用反應的重要角色，此種細胞表面有細菌脂多醣Lipopolysaccharide（LPS）的接受體，可利用LPS來誘發老鼠RAW 264.7巨噬細胞釋放一氧化氮（NO）；再以Griess reation 分析方法，測定NO含量變化，可以反映發炎之程度，並得以評估藥物對抗發炎過程的影響。黃芩的主成分為黃芩素及黃芩苷，本研究藉由NO釋放的效應，以體外試驗方式，進行化學成分之抗發炎作用探討。結果顯示，黃芩的50%乙醇粗抽物及其活性成分黃芩素和黃芩苷都具有抗發炎效果，其對抑制RAW 264.7巨噬細胞釋放NO的EC50 值分別為0.2mg/mL、28μg /mL 及22μg /mL。同時，黃芩素和黃芩苷對NO生成的抑制效果具有加成性。

關鍵字

黃芩 ； 黃芩素 ； 黃芩苷 ； 一氧化氮 ； 抗發炎 ； 巨噬細胞

並列摘要

Macrophages play an essential role in innate immunity and inflammatory responses. Its surface has bacterial lipopolysaccharide (LPS) receptors. The nitric oxide (NO) production which has induced in LPS-stimulated RAW 264.7 cells is quantitatively determined by the Griss reaction method. NO may reflect the degree of inflammation and provide a measure to assess the effect of drugs on the inflammatory process. Main components of Scutellaria Baicalensis Georgi are baicalein and baicalein-7-β-D-gluopyranosiduronic acid. In this study, we attempt to investigate the effect of these chemical constituents on anti-inflammation in vitro. The results show that they suppressed NO production in LPS induced RAW 264.7 cells and the EC50 values were 0.2mg/mL (50% ethanol crude extract), 28μg/mL(baicalein) and 22μg/mL(baicalein-7-β-D-gluopyranosi duronicacid), respectively. Simultaneously, baicalein and baicalein-7-β-D-gluopyranosiduronic acid have the additive effect to suppress NO production.

並列關鍵字

Scutellaria Baicalensis Georgi ； Baicalein ； Baicalein-7-β-D-gluopyranosiduronic acid ； Nitric oxide ； Anti-inflammation ； Macrophage