- 學位論文
中草藥萃取物與化妝品常用果酸類 化合物之抗菌交互作用研究
Study on the Antibacterial Interaction of Herbal Extracts and Commonly Used Acid-based Compounds in Cosmetics
摘要
由於化妝品中合成抗菌劑（如Methyl Peraben(M.P.)、Propyl Paraben(P.P.)）的過度使用，經常是造成皮膚過敏及刺激皮膚的重要因素。因此，尋找替代以及減少合成抗菌劑成為化妝品業界的重大課題。目前，國內、外已有學者研究醫藥用抗生素與植物成分的交互作用，但針對化妝品中的合成抗菌劑與植物萃取物的交互作用，則尚未有相關的報導。本研究以抑制大腸桿菌、痤瘡桿菌為指標，檢討中草藥廣藿香萃取物與化妝品中常使用的杏仁酸及合成抗菌劑之間的體外交互（協同、加成、拮抗）作用，期待作為中草藥化妝品添加抗菌劑種類與劑量之參考，以減少合成抗菌劑對人體皮膚的傷害。 本研究先將中草藥廣藿香以甲醇進行萃取，然後採用紙片擴散法分別進行0.1%、0.5%、1.0%、1.5%、2.0%等等不同濃度之廣藿香萃取物、杏仁酸、M.P.及P.P.單獨的抗菌實驗，求出四種試劑之抑菌圈面積-濃度線性方程式，以及0.1%、1.0%、2.0%等等不同濃度之廣藿香萃取物、杏仁酸、M.P.及P.P.兩兩合併的抗菌實驗，最後再以聯用指數（Combination Index, CI）來評估四種成份彼此間的交互作用。 本研究的實驗結果顯示，中草藥廣藿香分別與杏仁酸、M.P.及P.P.一起結合使用時，對於抗大腸及痤瘡桿菌的能力均具有良好的協同或加成作用。這意味著，化妝品中合成抗菌劑M.P.和P.P.的配方用量可加以減少，增加以天然成分的廣藿香和杏仁酸用量進行取代，而仍能維持化妝品的整體抗菌能力。此外依本研究的評估，對於有抗青春痘需求的化妝品，現今常用的杏仁酸成分還可加入較多的中草藥廣藿香成分予以取代，並以1.0 %廣藿香與0.1 %杏仁酸的合併方式最具抗痤瘡桿菌的協同作用。至於，化妝品中的合成抗菌劑成分M.P.與P.P.，本研究顯示這二種合成抗菌劑合併使用具有頗為明顯的抗菌協同作用，所以即使沒有加入中草藥等植物性萃取物成份，原有M.P.與P.P.的劑量亦可適度地予以降低。
並列摘要
Artificial antibacterial agents (such as Methyl Peraben(M.P.) and Propyl Paraben(P.P.)) were important factor in causing skin allergies and skin irritation. Therefore, searching for alternatives and decreases of the artificial antibacterial agents have become a major issue in cosmetics industry. The interactions between antibiotic medicines and plant components have already been investigated by domestic and foreign scholars. Nonetheless, there isn’t relevant reports that relative to interactions between artificial agents in cosmetics and the extracts of Chinese herbal. This study evaluates the in-vitro interactions (synergism, addition effect, antagonism ) between methanol extracts of Pogostemon cablin, Almond acid and two kinds of common ingredients used in cosmetics, against Escherichia coli and Propionibacterium acne. The results of this study were expected that as a reference of type and that dose for adding antibacterial agents in cosmetics and Chinese herbals in order to reduce damage to human skin. In this study, we extracted Pogostemon cablin with methanol, then practiced respectively the alone and one-on-one combined Antibacterial Susceptibility Testing (AST) of four kinds of reagents (Pogostemon cablin, almond acid, M.P. and P.P.) of vary concentrations (0.1 %, 1.0 %, 2.0 % and so on) with the Disk-diffusion method, and at last assessed the interactions between these four reagents with the Combination Index (CI). The results of this study showed that Pogostemon cablin possessed good synergism or addition effects when it was combined respectively with Almond acid, M.P. and P.P.. This means that the formula amounts of M.P. and P.P. can be reduced and substituted by the increased using of Pogostemon cablin extracts and Almond acid in cosmetics, and the overall antibacterial ability of cosmetics could still be able to maintain. In addition to assess for anti-pimples of cosmetics in dose study that Almond acid which is commonly used could be replaced by adding more ingredients of Pogostemon cablin, and the best combination formula is 1.0 % Pogostemon cablin to 0.1 % Almond acid. As for the components of combined artificial antibacterial agents, M.P. and P.P., this study indicated that there is obvious antibacterial synergism while combining these two artificial antibacterial agents. Thus, even without the addition of Chinese herbal extracts; the original amount of M.P. and P.P. can be moderately reduced.
參考文獻
延伸閱讀
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