This paper used frustrated total internal reflection configuration with Otto (gallium phosphide prism-peanut oil-silver metal film layer) excitation surface plasma wave measurements for peanut oil by different silver metal film layer thickness changes in computer simulation to find this Otto machine through a silver metal film layer optimal configuration film thickness of the surface plasma wave. This paper also completed the entire simulation results for in-depth analysis and discussion. The results of this study show the measuring of substances peanut oil of Otto configuration with silver metal film layer thickness of 330 (nm) has the best detection response. Namely, the present study configured in silver metal film layer thickness of 330 (nm) surface plasma waves produced deep and narrow optimal resonance angle of surface plasma wave.