- 期刊
瓊崖海棠與石栗種仁油之脂肪酸及功能性分析
Fatty acids and functional analysis of the seed oil from Calophyllum inophyllum and Aleurites moluccana
摘要
本省瓊崖海棠與石栗為少數栽植可做為生產種仁油的樹種，其種仁油都具有傳統醫療效果，但對兩者的種仁油之脂肪酸及功能性分析基礎研究尚缺。本實驗主要探討兩樹種種仁的收油率及分析主要飽和與不飽和脂肪酸成分及濃度，進而探討種仁油是否能影響傷口癒合及對細胞的毒性，最後探討瓊崖海棠種仁油分離的黃色素及綠色素造成細胞死亡的機轉。瓊崖海棠與石栗種仁的收油率會隨栽植地點及加工方式而改變，分別是在52.78-66.67%及66.3-69.6%間，兩種仁油皆含飽和脂肪酸及不飽和脂肪酸，其含量在瓊崖海棠是30.3%及69.7%，而石栗則是9.7%及89.6%。兩種仁油主要脂肪酸種類相同，但含量有差異。兩種仁油皆可促進傷口癒合，但隨濃度而有不同效果。低濃度瓊崖海棠種仁油會促進傷口癒合，隨濃度增加而降低傷口癒合效果，石栗種仁油則有完全相反傷口癒合效果。瓊崖海棠種仁油分離的綠色素較黃色素有較低的細胞毒性。在24小時處理後，人類細胞A549細胞會顯現晚期細胞凋亡現象，但隨色素濃度增加，早期和晚期細胞凋亡及細胞壞死比例亦發生改變。
並列摘要
C. inophyllum and A. moluccana are two of few plants for oil production in Taiwan. Although both seed oils have been reported as traditional medicines, the fatty acid composition and functional analysis of both seed oils is still scare. The aims of this study were to investigate the oil recovery, components and concentration of the saturated as well as unsaturated fatty acids in both seed oils, the wound healing and cell death of the both seed oils and yellow and green pigments of C. inophyllum to human A549 cells. The seed oil recivery of the seed were associated with plantation and manufacture methods. However, the oil recovery rate was 52.78-66.67% for C. inophyllum and 66.3-69.6% for A. moluccana. Both seed oil contained saturated and unsaturated acid with the rate of 30.3% and 69.7% for C. inophyllum and 9.7% 及 89.6% for A. moluccane, respectively. Although both seed oils could enhance the wound healing, reverse healing effect was dose- and species dependent. In C. inophyllum, increase of the seed oil concentration reduced the wound healing. On the contrary, the seed oil of A. moluccana demonstrated the reverse phenomenon. Green pigment from the seed oils of the C. inophyllum exhibited lower cytotoxicity to human A549 cells than the yellow pigments. After treating 24 hours with pigments both pigments induced mainly late apoptosis. However, increase of the concentration changed the ratio of early and late apoptosis and necrosis.
並列關鍵字
Calophyllum inophyllum ； Aleurites moluccana ； Fatty acid ； Wound healing ； Apoptosis ； Pigment延伸閱讀
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