Taiwan has been well known internationally as the "Kingdom of Phalaenopsis", and exports orchids worldwide. In vitro aseptic germination and micropropagation of orchids are key propagation system for orchid commercial production. Regarding the methodologies to asymbiotic germination and its regeneration are explored. This paper also discusses the rapid mericlonal multiplication techniques and general background information used in micropropagation studies. We expect that the protocols provides an overview on various orchids micropropagation used in mass production.