The Effects of Brussels Sprouts and Their Components, Indole-3-Carbinol (I3C) and l-Cyano-2-HydroxY-3-Butene (CHB) on Hepatic Detoxification Enzyme Activitles

抱子甘藍和其成份物I3C和CHB對肝臟解毒酵活性調節之探討

吳宏乾(Hung-Chien Wu) ； 林昭庚(Jaung-Geng Lin) ； 張永賢(Yung-Hsien Chang) ； 黃維三(Wei-San Huang) ； 蔡宗博(Tsung-Po Tsai) ； 余榮敏(Jung-Min Yu) ； 劉忠榮(Chung-Jung Liu) ； 黃獻平(Hsien-Ping Huang) ； 黃雅慧(Ya-Hui Huang) ； 劉哲育(Jer-Yuh Liu) ； 郭薇雯(We)

《Mid-Taiwan Journal of Medicine》 7卷1期 (2002/03) Pp. 28-37

https://doi.org/10.6558/MTJM.2002.7(1).5

摘要

背景 屬於十字花科的抱子甘藍,無論是在動物或人體實驗都顯示有預防癌症效果而此機轉是由於抱子甘藍能夠刺激致癌物代謝的解毒酵素活性,然而是否抱子甘藍的主要成份13C及CHB主導了解毒醉素活性之產生,則尚待驗證。
方法 質驗分成兩部份來進行。抱子甘藍之冷凍乾燥粉末以飼料重量20%含率餵以大 白鼠及小白鼠7天,並以控制相同攝取量為對照組,在大白鼠及小白鼠實驗中,和對照 組比較。
結果 無論解毒酵素phase I '如CYPIA '及phase II '如GST及QR'活性皆顯著 上升,在進一步之實驗中,以1% BHA為正控制組, 6.25 mg 13C/g及4 mg CHB/g 為實驗組。結果顯示,肝臟中GSH含量在任何一組皆無明顯活性上升。13C則刺激 GST, QR及CYPIA活性上升,分別為對照組之2.4 ' 2.4及5.6倍,但是CHB並無任何顯著效果。
結論 我們認為抱子甘藍防癌效果,可能(至少部分)是來自於成份13C'非CHB之對 解毒酵素活性的刺激所造成。(中台灣醫誌2002;7:28-37)

關鍵字

抱子甘藍 ； 肝臟解毒酵素 ； I3C ； CHB

並列摘要

Background. Brussels sprouts, one of many cruciferous vegetables, have been shown to have chemoprevention effects in both animal and human studies. The mechanism of chemoprevention activity is associated with the induction of drug-metabolizing enzymes, which modulate carcinogen metabolism. Whether indole-3 carbinol (I3C) and l-cyano-2 hydroxY-3-butene (CHB), two major components in Brussels sprouts, contribute to the inductions of drug-metabolizing enzymes has not been determined yet. Therefore, the chemopreventive effects of Brussels sprouts and their components were separately investigated in 2 experiments, by our group.
Methods. Freeze-dried Brussels sprouts (BS) powder at 20% diet (w /w) was fed to mice and rats (4/group) for 7 days. Pair-fed animals were used as the control group.
Results. Significant inductions were shown in both phase II enzymes, such as glutathione Stransferase (GST) and quinone reductase (QR), and phase I enzymes, such as cytochrome P450。lA (CYP IA), in the mouse-and rat groups compared to .their pair-fed groups, In the second study, the treatment diet included 6.25mg 13C/g diet, 4 mg CHB/g diet, and 1% butylated hydroxyanisole (BHA) as a positive control.
Conclusions. No significant glutathione (GSH) induction was seen in any treatment group.13C produced significant induction in GST, QR and CYPIA which were 2.4-, 2.4-,and 5.6-fold, respectively, over the control group. However, CHB did not show any significant inductive effects. Therefore, we concluded that 13C, not CHB, at least in part, contributes to the chemopreventive effect of inducing drug-metabolizing enzymes in Brussels sprouts. ( Mid Taiwan J Med 2002;7:28-37)

並列關鍵字

brussels sprouts ； detoxification enzymes ； indole-3-carbinol (I3C) ； l-cyano-2-hydroxy-3-butene (CHB)