轉型生長因子－β在口腔粘膜下纖維性病變之免疫組織化學表現

Immunohistochemical Expression of Transforming Growth Factor βin Oral Submucous Fibrosis

黃志浩(Chih-Hao Huang) ； 謝天渝(Tien-Yu Shieh)

《臺灣口腔醫學會雜誌》 15卷2期 (1999/12) Pp. 227-239

摘要

口腔粘膜下纖維性病變是一種發生在口腔粘膜的慢性疾病，其主要特徵為口腔粘膜下膠原纖維異堆積及表皮萎縮。更有學者視之為口腔鱗狀上板細胞癌之癌前病變。TGF-β是多胜類，其特有的細胞效應可刺激造纖維細胞使合成膠原纖維蛋白及細胞外基質、抑制膠原纖維蛋白酵素而促使膠原纖維與細胞外基質堆積。許多身體上的纖維性疾病如肝硬化、間質性肺纖維化、腎絲球體腎炎及纖維性皮膚病變已知與它有關。本實驗使用免疫組織化學染色方法來標定出TGF-β存在於正常口腔粘膜及口腔粘膜下纖維性病變的位置，嘗試尋找出組織病理上之潛在差異，以提供做未來進一步實驗之依據。結果，在粘膜下的結締組織層，口腔粘膜下纖維性病變後期患者的口腔粘膜其TGF-β表現與正口腔粘膜之間並無明顯差異，但病變前患者的口腔粘膜其TGF-β表現明顯高於正常口腔粘膜。因此推論，TGF-β與口腔粘膜之轉變為纖維化可能有十分密切的關係。

關鍵字

口腔粘膜下纖維性病變 ； 轉型生長因子－β ； 免疫組織化學染色

並列摘要

Oral submucous fibrosis (OSF) is characterized by an abnormal accumulation of ollagenfibers in oral submucosa and is also regarded as a precancerous lesion. Evidence of fibroblast
proliferation, increased collagen formation and decreased activity of collagenase in OSF have been described by many authors. Transforming growth factor beta (TGF-beta), a 25Kd polypeptide of 112 amino acids, is a multi-functional growth factor that reg ufates
the cell growth, differentiation and extra-cellular matrix formation (ECM) in both normal and transformed cells. Increased activation of TGF-beta can result fibrogenic diseases such as liver cirrhosis, interstitial pulmonary fibrosis, glomerulo-nephritis, and skin fibrotic disease. This has been described due to the effects of TGF-beta stimulatation of the depositon of collagen and other matrix components by fibroblasts; inhibiton of of collagenase; locking of plasminogen inhibitor; enhancement of angiogenesis. Furthermost, it is due to the chemotaxis for fibroblasts, monocytes, and macrophages In this study, immunohistochemical staining was used to localiz and to find the differences of expression of the TGF-beta between clinical normal oral mucosa and mucosa of OSF. The results indicate that there is no difference over submucosal area between normal specimen and those from late stages of OSF. But the tissues from inflammatory lesions and early stage of OSF demonstrate higher immunohistochemical expression of TGF-beta. We conclude that TGF-beta may playa role in the OSF similar to other fibrogenic diseases.

並列關鍵字

Oral submucous Fibrosis(OSF) ； Transforming GrowthFactor-β(TGF-β) ； Immunohistochemistry