應用豬卵體外受精技術評估公豬精液保存效果

The Use of In Vitro Fertilization of Pig Oocytes to Assess Fertilizing Ability of Stored Boar Semen

陳銘正(Ming-Cheng Chen) ； 陳逸仲(I-Chung Chen) ； 陳立峰(Li-Fong Chen) ； 陳維泰(Wei-Tay Chen) ； 劉淑屏(Su-Ping Liu) ； 方瓊郁(Chiung-Yu Fang) ； 林佳靜(Chai-Ching Lin)

《宜蘭技術學報》 2期 (1999/06) Pp. 19-24

https://doi.org/10.6179/nit.1999.02.13

摘要

本試驗之目的，在應用豬卵母細胞之體外成熟與體外受精技術，比較公豬精液稀釋保存之效果，期能在短時間內鑑定稀釋精液之受精能力變化。本試驗係以手握法採取公豬新鮮精液，經添加Modena稀釋液後，保存於15℃恆溫冰箱中八日。試驗期間測定原精液及稀釋精液之pH值，並分別檢查其活力及頭巾完整性，最後進行體外受精，評估精子之受精率，俾與精液保存期間精液品質變化情形相互比較。試驗結果顯示，稀釋精液在0至8日之保存期間，pH值維持在6.96至7.13之間，而原精液在同一期間之pH值則由7.32降至5.97。原精液與稀釋精液於保存二日後，稀釋精液內精子活動率顯著高於原精液者；稀釋精液被保存至第六日時，精子仍保有60%以上之活動率，原精液組則於第四日急遽降至37.5%。原精液保存四日後，精子具不完整頭巾之百分率則由2.55%提高至48%，第六日以後則高達90%以上；稀釋精液組則在保存四日內頭巾不完整百分率仍維持在8.8%水平內，惟第六日後才被提高至22.5%。應用前述不同保存期間之稀釋精液，在體外完成獲能作用後進行體外受精試驗，結果證明公豬新鮮精液添加Modena稀釋液後保存2日者可維持78.43%之高受精率，惟當保存期延長至4-5日或6-7日時，則受精率分別降低至44.3%與9.9%。

關鍵字

公豬 ； 精液保存 ； 體外受精

並列摘要

The purpose of this study was to use in vitro fertilization (IVF) techniques for assessing the fertilizing ability of boar sperm that was stored for 8 days. Semen was collected by gloved-hand method, diluted with Modena extended and stored in a refrigerated incubator at 15℃. Stored semen was evaluated for pH value, sperm motility, acrosome integrity and subsequently used for in vitro fertilization. Results showed that pH value of diluted semen was stable and maintained from 6.96 to 7.13 during storage, however that of undiluted semen was significantly decreased from 7.32 to 5.97. The semen preserved in extender obtained higher motility than undiluted semen after 2 days storage. The sperm motility of diluted semen was still over 60% at day 6, but that was decreased to 37.5% at day 4 in undiluted semen. The percentage of abnomal acrosome spermatozoa was still under 8.8% in diluted semen stored within 4 days, but that was increased to 22.5% at day 6. Meanwhile, the abnormal acrosome rate of spermatozoa was increased to 48% at day 4 and over 90% after 6 days storage in undiluted semen. Further results from IVF assay revealed that boar sperm that had been previously stored for 2 days in Modena extender fertilized 78.43% of porcine oocytes. However, the increase of storage time to 4-5 days and 6-7 days would result in a much decrease of fertilization rate to 44.3% and 9.9%, respectively.

並列關鍵字

Boar ； Semen preservation ； In vitro fertilization