黃麴毒素B1對小鼠及其腹腔巨噬細胞毒害之影響

Effects of Aflatoxin B1 Toxicity on Murine and It's Peritoneal Macrophages

鄭永祥(Yeong-Hsiang Cheng) ； 張銘煌(Ming-Huang Chang) ； 李德南(Der-Nan Lee) ； 朱惠君(Huei-Chun Chu) ； 江映紅(Ying-Hung Chiang)

《宜蘭技術學報》 8期 (2002/06) Pp. 21-28

https://doi.org/10.6179/nit.2002.08.13

摘要

本實驗之目的是探討小鼠經黃麴毒素B1（aflatoxin B1, AFB1）攻毒後，對小鼠毒害及其腹腔巨噬細胞存活率、一氧化氮（nitric oxide, NO）分泌之影響和誘發細胞凋亡之觀察。小鼠經不同濃度AFB1（0、200、400及800μg/Kg BW）口服攻毒兩週。犧牲後取臟器及血液，進行組織切片及血液生化分析。結果顯示，巨噬細胞經不同濃度AFB1攻毒，對小鼠血球容積比與收穫巨噬細胞數於各處理組間無顯著差異。AFB1對隊小鼠腎、脾、心臟及睪丸之組織病理學檢查，結果顯示均無特異性病變產生，但對肝臟組織檢查時，可見肝細胞空泡化、核腫脹局部肝細胞變性或壞死及膽管增生。在血液生化方面，僅天門冬胺酸轉胺（Asparate aminotransferase, AST）於AFB1 200及800μg/Kg BW組顯著較對照組為高。巨噬細胞於體外以不同濃度AFB1（0、5、10、20及30μg/mL）共同培養後，進行細胞存活率及NO分泌量之測定及誘發細胞凋亡之觀察。結果顯示，隨作用時間及毒素濃度提高其細胞存活率隨之下降。當巨噬細胞經AFB1攻毒，結果顯示3小時攻毒時隨著AFB1濃度自10μg/mL，NO分泌下降（p＜0.05），相同之NO分泌抑制結果亦見於6及12小時處理者。DNA電泳結果發現，巨噬細胞無論有無AFB1攻毒均可發現DNA階梯狀片段之情形。

關鍵字

黃麴毒素B1、毒害、巨噬細胞、小鼠

並列摘要

The purpose of this study was to investigate the effects of aflatoxin B1 (AFB1) on murine toxicity, cell viability, secretion of nitric oxide, and induction of apoptosis by in vivo and in vitro challenge. Mice were orally challenged by different concentrations of AFB1 (0, 200, 400, and 800μg/Kg BW) for 2 weeks. Histopathology alterations of visceral organs and changes of blood biochemistry assays were evaluated after mice were sacrificed. The results showed that there were no significant difference in percentage of packed cell volume and number of harvested peritoneal macrophages among treatments. No specific lesions were observed in several visceral organs, except liver. The section examination revealed hepatocyte vaculolization, nucleus swelling, local necrosis and bile duct proliferation. In biochemistry assays, AFBI 200 and 800μg/Kg BW treatments had significantly higher asparate aminotransferase (AST) activity than control group.Macrophages were cocultured with AFBI (0, 5, 10, 20 and 30 ug/mL). Cell viability, secretions of nitric oxide and induction of apoptosis were evaluated. The result showed that cell viability was decreased along with the culture time and dosage of AFB1. Secretion of nitric oxide declined (P＜0.05) after the dosage of AFB1 was increased from 10 μg/mL to 30μg/mL at the time point of 3 hr challenge, and similar nitric oxide inhibition was also observed in 6 and 12 hr challenge. There were same DNA ladder patterns of macrophages observed with or without AFB1 challenged for 12 hr. This result revealed that apoptosis occurred both in control group and AFB1 treatment.

並列關鍵字

Aflatoxin B1 ； Toxicity ； Peritoneal macrophages ； Murine