Unveiling the Prediction Value and Immunological Role of miR-29b in Breast cancer via Bioinformatic Analysis

通過生物資訊學分析揭示miR-29b在乳癌的預測價值和免疫學作用

Hung-Yu Lin(林宏昱) ； Hsiu-Hung Huang(黃琇紅) ； Pei-Yi Chu(朱旆億)

《秀傳醫學雜誌》 預刊文章 Pp. 1-22

https://doi.org/10.30185/SCMJ.202212/PP.0031

摘要

Breast cancer (BC) is the most prevalent malignant tumor and one of the main causes of tumor-related death around the world. The tumor immune microenvironment (TIME) plays a vital role in cancer progression. Using bioinformatic data source, we found that high miR-29b was associated with longer overall survival time and that BC tumor expressed higher miR-29b than normal tissue. Using single cell transcriptomes database, we revealed that miR-29bprimarily expressed in cells that mediate T cell cytotoxicity, including NKT, NK, CD8+ naïve T cell and CD4+ naïve T cells. Using the combination of miRDIP database with Metascape algorithm, we showed that miR-29b-targeted genes predominantly mediate collagen formation. Using TansmiR v2.0 database along with ENCORI platform, we identified IRF1 as a potential transcription factor driving the expression of miR-29b in human BC. In conclusion, high miR-29b serves as a prediction factor for favorable survival in BC patients. miR-29b expresses primarily in cells that mediates T cell cytotoxicity. Interferon regulatory factor 1 (IRF1)serves as a potential transcription factor driving miR-29b and the resultant pathway predominantly lies in collagen formation. These findings provide an opportunity for the development of a new diagnostic/prognostic biomarker and therapeutic approach targeting miR-29b-associated network.

關鍵字

Breast cancer ； miR-29b ； Prediction value ； Collagen formation ； Tumor immune microenvironment

並列摘要

乳癌是全球發病率最高的惡性腫瘤，也是全球腫瘤相關死亡的主要原因之一。腫瘤免疫微環境在癌症進展中起著至關重要的作用。使用生物資訊學數據源，我們發現高miR-29b與較長總生存時間相關，並且乳癌腫瘤比正常組織表達更高的miR-29b。使用單細胞轉錄組數據庫，我們發現miR-29b主要在介導T細胞細胞毒性的細胞中表達，包括自然殺手T細胞、自然殺手細胞、CD8+初始T細胞和CD4+初始T細胞。使用miRDIP數據庫與Metascape演算法的組合，我們發現miR-29b靶向基因主要介導膠原蛋白的形成。使用TansmiR v2.0數據庫和ENCORI平台，我們鑑識IRF1為驅動人類乳癌miR-29b表達的潛在轉錄因子。總之，高miR-29b可作為乳癌患者良好生存的預測因素。miR-29b主要在介導T細胞細胞毒性的細胞中表達。IRF1作為驅動miR-29b的潛在轉錄因子，產生的途徑主要在於膠原蛋白的形成。這些發現為開發針對miR-29b相關網絡的新診斷/預後生物標誌物和治療方法提供了機會。

並列關鍵字

乳癌 ； miR-29b ； 預測價值 ； 膠原蛋白形成 ； 腫瘤免疫微環境