多花型菊花總花托、癒傷組織以及懸浮培養細胞照射γ射線後之再生

Plant Regeneration from Capitulum, Callus and Suspension Culture of Chrysanthemum (Dendranthema × grandiflorum (Ramat) Kitamura) after γ-ray Irradiation

江純雅(Chun-Ya Chiang) ； 朱建鏞(Chien-Young Chu)

《植物種苗》 8卷3期 (2006/09) Pp. 19-28

https://doi.org/10.30077/SN.200609.0002

摘要

菊花'Monami'、‘粉火焰’、‘小乒乓’、‘紅風車’之葉片和花托之癒傷組織或花托培養於固體培養基，再分別以4 Gy劑量之γ射線照射一、二、三次。照射後的'Monami'和‘粉火焰’培殖體以花托再生不定芽之比率較高。而‘小乒乓’培殖體僅花托可再生不定芽。經照射4Gy兩次者，不定芽再生芽體數顯著低於照射4Gy一次或三次者。三品種皆產生白花變異株。‘紅風車’液體懸浮培養之癒傷組織照射γ射線後形成不定芽非常慢。照射4Gy兩次者，在培養35週後僅得到24個芽。在懸浮細胞再生株中可發現不同花序排列的變異株。

關鍵字

γ射線 ； 花托培養 ； 懸浮培養 ； 育種 ； 變異

並列摘要

Calli derived from leaf and capitulum, and capitulum of chrysanthemum 'Monami', 'Pink Flame', 'Little Pin Pon' and 'Red Windmill' were cultured on a solified medium. There explants were irradiated 4 Gy γ-ray for 1, 2 or 3 times, respectively. The proliferation rate of adventitious shoots from the capitulum explants of 'Monami' and 'Pink Flame' capitulum was higher than that of other explants. In addition, shoots only regenerated from 'Little Pin Pon' capitulum explants. The regenerated shoots from explants irradiated 4 Gy γ-ray twice were fewer than that from explants irradiated once or three times. The mutants from three cultivars with white flowers were found. The 'Red Windmill' regeneration of suspension cells irradiated was very slow. Suspension cells after haven irradiated 4 Gy γ-ray twice proliferated 24 adventitious shoots after 35 weeks. The mutants with different inflorescence arrangement were found in the regenerated plants from suspension cells.

並列關鍵字

γ-ray ； capitulum culture ； suspension cell ； breeding ； mutation