本研究以具丑斑(harlequin type)與不具丑斑花色的蝴蝶蘭進行雜交,探討蝴蝶蘭花朵丑斑表現型的遺傳行為。結果顯示,花朵不具丑斑表現型的Phalaenopsis Sunrise Red Peoker‘#1’和Phal. Brother Stage‘#1’兩親本,其正反交子代的花朵表現型皆不具丑斑。而花朵表現型為丑斑的Phal. Sunrise Red Peoker‘#2’親本,分別與不具丑斑表現型的Phal. Brother Stage‘#1’、Phal. I-Hsin Peacock ‘KHM063’、Phal. Ching Her Buddha‘KHM128’及Phal. Fashion Little Star‘#1’雜交,其子代花朵具丑斑與不具丑斑的植株比率,分別為99:100、24:19、87:101及11:16,其結果接近預期的1:1基因分離比。當Phal. Sunrise Red Peoker‘#2’與其母本Phal. Golden Peoker‘BL’回交時,其子代花朵具丑斑與不具丑斑的植株比率48:15,結果接近預期的3:1基因分離比。採用卡方法測驗假說的結果顯示,蝴蝶蘭花朵的丑斑表現型相對於不具丑斑者為顯性,且可能是由單一基因座所控制。
To study the inheritance of harlequin trait, Phalaenopsis orchids were used as parents for crossing materials. The results showed that hybrids from reciprocal crosses between Phalaenopsis Brother Stage '#1' and Phal. Sunrise Red Peoker '#1' (both non-harlequin types) were all non-harlequin type. However, the hybrids from crosses between harlequin type (Phal. Sunrise Red Peoker '#2') and non-harlequin type (Phal. Brother Stage '#1', Phal. I-Hsin Peacock 'KHM063', Phal. Ching Her Buddha 'KHM128' and Phal. Fashion Little Star '#1') showed 99:100, 24:19, 87:101 and 11:16 ratios of harlequin type to non-harlequin type, respectively. These results approximate the expected genetic segregation ratio of 1:1. When Phal. Sunrise Red Peoker '#2' adopted as a male parent and back crossed to Phal. Golden Peoker 'BL', hybrids produced 48:15 ratio of harlequin type to non-harlequin type. This result approximated the expected genetic segregation ratio of 3:1. Results suggested that harlequin type of Phalaenopsis flower was dominant to non-harlequin type, and might be genetically determined at a single locus. The hypotheses were tested using the chi-square method.