紅鶴頂蘭花梗節芽培養與增殖

Stalk Node Bud Culture and Multiplication of Phaius Tankervilleae

蔡雅竹(Ya-Chu Tsai) ； 張正(Chen Chang)

《植物種苗》 14卷2期 (2012/06) Pp. 31-40

https://doi.org/10.30077/SN.201206.0003

摘要

以四種長度的紅鶴頂蘭花梗之頂芽、花芽及營養芽做為初代培養之培植體，長度小於5cm花梗的頂芽培植體具有66.7%芽體形成率。長度11-15cm花梗的花芽及營養芽培植體的芽體形成率分別為7.1%及10.9%。源自於花芽及營養芽生成的芽體除葉及除根後增殖六代，每代增生1.3-1.9倍芽體，芽體移入發根培養基2個月後移植出瓶。小苗栽培於溫室兩個月，不同培植體來原的小苗存活率介於75%-87.5%之間。

關鍵字

紅鶴頂蘭 ； 花梗節芽 ； 增殖

並列摘要

Various lengths of flower stalks were used for in vitro propagation of Phaius tankervilleae as explants. Apical flower buds, flower buds, and vegetative buds in flower stalks were taken as explants to establish in vitro cultures. The results showed that the length short 5 cm flower stalks had a 66.7% shoot formation, and was a record in apical flower buds. However, the highest shoot formation rate was for flower buds and vegetative buds, respectively. Two types of explants, derived from flower buds or vegetative buds, were used for consecutive multiplication experiments. Explants were removed from their leaves and roots before monthly subculture and a 1.3-1.9 times shoot proliferation rate was obtained. Explants were transferred to a rooting medium for 2-mo culture. Plantlets were then deflasked in a greenhouse for 2-month culture. There was a 75%-87.5% plantlet survival rate that was derived from different original explants.

並列關鍵字

Phaius tankervilleae ； stalk bud ； multiplication