辣椒花粉保存之研究

Study on Pollen Preservation of Hot Pepper (＂Capsicum annuum＂ L.)

郭宏遠(Horng-Yeuan Kuo) ； 宋妤(Yu Sung)

《植物種苗》 16卷3期 (2014/09) Pp. 1-18

摘要

本研究採不同溫度進行辣椒花粉保存以瞭解其效果，並進行液態氮保存之相關研究，探討其應用於種原保存、育種及採種之可能性。試驗結果顯示室溫下花粉只能維持3天活力，在4℃下花粉活力隨保存時間持續下降，9週後已無發芽能力，而授粉後之單果種子數及種子發芽率則在4週後就明顯下降。於-20℃下之花粉發芽率在第12週時開始明顯降低，而液態氮保存12週後仍保有活力，此兩處理對授粉後之單果種子數及種子發芽率均無影響。液態氮回溫條件以40℃水浴回溫處理3-5分鐘為適當，保存1年後仍能保持其活力。此法可提供長期之保存效果，有助於蔬菜育種及採種之應用。

關鍵字

辣椒 ； 花粉活力 ； 超低溫保存

並列摘要

The effect of the different temperatures on pollen storage and the probability of applying cryopreservation on germplasm preservation, breeding and seed production of hot pepper was researched in this study. The results showed that pollen could only maintain vigor for three days in room temperature, and the vigor of pollen storaged in 4℃ condition consistently declined until the ninth week, when no germination was obtained, however, the seeds per fruit and seed germination percentage decreased markedly when in the fourth week. In addition, the pollen germination ability started to decline in the twelfth week in -20℃. While no decrease was observed in cryopreservation treatment, the seeds per fruit and seed germination percentage of both treatments were still the same with fresh pollen treatment. Furthermore, 40℃ water bath for 3-5 min was suitable for thawing after pollen cryopreservation. The pollen cryopreserved for one year could maintain its vigor. It was suggested that cryopreservation was useful for long-term pollen storage and helpful for application on vegetable breeding and seed production.

並列關鍵字

Hot pepper ； pollen viability ； cryopreservation