未加鈣系統溶液中明膠與褐藻膠間之交互作用

Interaction between Gelatin and Algin in No-calcium-added Solution

王裕泰(Yuh-Tai Wang) ； 張爲憲(Wei-Hsien Chang)

《臺灣農業化學與食品科學》 42卷6期 (2004/12) Pp. 448-455

https://doi.org/10.6578/TJACFS.2004.057

摘要

The interaction of gelatin (G) and algin (A) was affected by the pH of the solution, individual and total concentrations as well as G/A ration in a no-calcium added system was studied in this experiment. No matter how the G/A ratio changes in the G-A solution, the turbidity reached a peak value when the pH of interaction between G and A decreased. Under a fixed 0.005% of G, the peak-turbidity of G-A solution decreased with the increasing concentration of A; under a fixed 0.001% of A the peak-turbidity increased with the increasing concentration of G; however, if the total concentration (A+G) was fixed at 0.01%, the peak-turbidity would increased first and then decreased with the increasing of 0 concentration or the value of G/(G + A) and the turning point was around 0.8 of G/(G+A) or 4.0 of G/A. In addition, with a fixed G/A, the peak-turbidity went straight up when the total value of (G+A) increased and the straight up slope changed with the G/A ratio, nevertheless, increased and then dropped with the increasing value of G/(G+A). Also, the turning point of the slope was 0.8 of G/(G+A) or 4.0 of G/A ratio and that is to say, the type of interaction between G molecule and A molecule changed as well. In comparing the influence caused by adding 0.2 M NaCl and 1-8 M urea or not on the interaction of G and A, the result shown that: In the condition of pH＞3.5, the interaction of G and A was mainly the ionic bond, and then the hydrogen bond followed by hydrophobic interaction; under the condition of pH＜3.0-3.5, the major interaction of G molecule and A molecule was hydrogen bond. When G/A ratio changed the pH had to be adjusted as well in order to maintain the relative ratio of total net charge for G-A solution to be at the most beneficial condition for interaction.

關鍵字

明膠 ； 褐藻膠 ； 褐藻酸 ； 交互作用

並列摘要

The interaction of gelatin (G) and algin (A) was affected by the pH of the solution, individual and total concentrations as well as G/A ration in a no-calcium added system was studied in this experiment. No matter how the G/A ratio changes in the G-A solution, the turbidity reached a peak value when the pH of interaction between G and A decreased. Under a fixed 0.005% of G, the peak-turbidity of G-A solution decreased with the increasing concentration of A; under a fixed 0.001% of A the peak-turbidity increased with the increasing concentration of G; however, if the total concentration (A+G) was fixed at 0.01%, the peak-turbidity would increased first and then decreased with the increasing of 0 concentration or the value of G/(G + A) and the turning point was around 0.8 of G/(G+A) or 4.0 of G/A. In addition, with a fixed G/A, the peak-turbidity went straight up when the total value of (G+A) increased and the straight up slope changed with the G/A ratio, nevertheless, increased and then dropped with the increasing value of G/(G+A). Also, the turning point of the slope was 0.8 of G/(G+A) or 4.0 of G/A ratio and that is to say, the type of interaction between G molecule and A molecule changed as well. In comparing the influence caused by adding 0.2 M NaCl and 1-8 M urea or not on the interaction of G and A, the result shown that: In the condition of pH＞3.5, the interaction of G and A was mainly the ionic bond, and then the hydrogen bond followed by hydrophobic interaction; under the condition of pH＜3.0-3.5, the major interaction of G molecule and A molecule was hydrogen bond. When G/A ratio changed the pH had to be adjusted as well in order to maintain the relative ratio of total net charge for G-A solution to be at the most beneficial condition for interaction.

並列關鍵字

Gelatin ； Algin ； Alginic acid ； Interaction