以溶膠-凝膠法製備鈣磷矽混合物

Study of Biocompatible Ca-P-Si Composites Prepared Through a Sol-gel Process

黃士維(Shih-Wei Huang)

指導教授 ： 鍾仁傑

國立臺北科技大學/工程學院/生物科技研究所/碩士(2010年)

https://doi.org/10.6841/NTUT.2010.00316

摘要

本研究目標為以溶膠凝膠法製備鈣磷矽系混合物，主要無機成分為三鈣矽酸鹽(Ca3SiO5, tricalcium silicate, C3S)及氫氧機磷灰石(Ca10(PO4)6(OH)2, hydroxyapatite, HAp)， C3S有良好機械性質及自固性、HAp有良好生物相容性，本研究主要探討在C3S中添加適當HAp使其能兼顧生物相容性及抗壓強度。壓錠後之材料於SBF中以評估其離子釋出特性並以XRD、SEM做分析、機械以及物化性質由奈米壓痕試驗機、MTS、FT-IR、TG-DTA量測，由MTT assay與流式細胞儀評估其生物相容性，且以螢光顯微鏡、SEM觀察細胞貼附於材料上之生長情況。
在本研究結果可成功的減少在C3S、HAp合成過程所分解的CaO、使HAp/C3S粉末均勻混合且將粉體粒徑控制在20μm以下。合成之材料浸泡SBF 7天後皆能誘導磷灰石之生成，鈣矽磷混合物能將pH值控制在7.54至8.76之間。材料之浸泡液能將細胞增生率提高到96.3 %至136.6 %，沒有明顯地細胞毒性與細胞凋亡現象。以L929和MG-63細胞培養於C-75(75%C3S、25%HAp)材料與醫療級HAp上皆能完整的伸展且增生且C-75材料之抗壓強度為7.28 MPa。由奈米壓痕硬度機所量測C-75之硬度與楊式模數分別為2.69 GPa與59.18 GPa。C-75材料有良好生物相容性以及相對較高之抗壓強度，且可以均勻塗布於明膠薄膜上，未來可應用於生物高分子表面之鍍膜。

關鍵字

骨引導性 ； 鈣矽磷混合物 ； 溶膠凝膠法 ； 自固化作用 ； 低溫鍍膜

並列摘要

In this study Ca-P-Si composites were fabricated through the sol-gel processes.
The major crystalline phases of the composites were tricalcium silicate (C3S) and
hydroxyapatite (HAp). C3S is able to self-setting and with high compressive strength,
and HAp is well known for excellent biocompatibility. The designation of the
composites was to improve the biocompatibility of C3S through HAp addition.
Mechanical and physico-chemical properties were examined using nano-indentation,
material test system (MTS), fourier transform infrared and thermal gravity-differential
thermal analysis. In vitro studies were carried out using MTT assay and flowcytometer.
Fluorescent microscopy and SEM were used to observe the cell proliferation and
attachment behavior.
The results showed that there were no undesirable free CaO through the sol-gel
preparation. Well mixed HAp/C3S powders (>20μm) were achieved. Apatite-like layer
precipitated onto the composites after soaking the composites in simulated body fluid
(SBF) and the pH values were measured between 7.54 and 8.76. For in vitro study,
proliferation rate ranged from 96.3 % to 136.6 % for different composites. There were
no significant cytotoxicity and apoptosis phenomena observed for the Ca-P-Si
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composites. MG-63 and L929 cells attached onto C-75 (75%C3S, 25%HAp) spread as
well as medical grade HAp. Compressive stress of C-75 was 7.28 MPa. The average
hardness and young’s modulus obtained using nanoindentation for C-75, which were
2.69 GPa and 59.18 GPa, respectively. C-75 has good biocompatibility, high
compression stress and can be uniformly coated onto gelatin film. It is progressive for
further investigation as coating for biopolymers.

並列關鍵字

Osteo-inductive ； Ca-Si-P Composites ； Sol-gel Processes ； Self-setting ； Low Temperature Coating

參考文獻

Author(s): Wang XH, Sun HC, Chang J

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