溶膠凝膠法製備鈣矽磷/幾丁聚醣 複合材之特性研究

Characterization of sol-gel derived calcium silicon phosphate/chitosan composites

鄭人華(Zen-Hua Cheng)

指導教授 ： 丁信智

中山醫學大學/口腔醫學院/口腔材料科學研究所/碩士(2006年)

https://doi.org/10.6834/CSMU.2006.00016

摘要

氫氧基磷灰石(HA)是是人體骨組織成份，有良好的生物活性應用於人體骨組織修復已經有很長的歷史。矽元素被證實能增加材料在人體的生物活性，鋅元素則能夠抑制蝕骨細胞作用與刺激骨母細胞生成。幾丁聚醣對於傷口復原和骨組織再鈣化也有不錯的效果。綜合上述各材料之優點，本篇研究利用溶膠凝膠法合成含矽之之氫氧基磷灰石，並添加鋅元素製作成塊材，研究矽、鋅元素對氫氧基磷灰石物理、化學及機械性質的影響。同時也評估幾丁聚醣高分子滲入矽鋅磷灰石塊材的作用。
在溶膠凝膠製程中以硝酸鈣、TEP做為氫氧基磷灰石鈣磷的前驅物，以TEOS、氧化鋅做為矽、鋅元素來源，經過調整溶膠酸鹼值、熟化，120℃乾燥後得到乾燥膠體。結果顯示高的溶膠酸鹼值製作的粉末，在低溫300℃即可生成HA，而未調整溶膠酸鹼值的HA0組別則需500℃熱處理才能得到HA。
X光繞射分析中得知，隨著矽添加量提高使得氫氧基磷灰石的結晶相趨向非晶質，1 wt%氧化鋅也使得氫氧基磷灰石趨向非晶質，並且影響氫氧基磷灰石002的繞射峰顯示晶粒變小的結果。
機械強度分析的結果方向，矽的含量對塊材徑向拉伸強度有不利的影響，且含量達到Si4組別時急據降低，但以高的溶膠酸鹼值製作的塊材則可以避免這樣子的現象，這可能和pH 9組別有較大的密度有關。在生物活性方面，在經過模擬體液的浸泡後，幾丁聚醣覆蓋對於氫氧基磷灰石的表面活性有增進的效果，且在浸泡期間發現材料表面孔洞的形成顯示含矽鋅氫氧基磷灰石/幾丁聚醣複合材為生物降解性、生物活性、骨引導性之優良材料。

關鍵字

溶膠凝膠法 ； 矽 ； 氫氧基磷灰石 ； 幾丁聚醣

並列摘要

Hydroxyapatite (HA) ceramics are currently used as bone graft substitutes because of its osteoconductivity. Silicon could enhance the bioactivity of materials. Zinc improves quantity and quality of osteoblast cells and acts as a stimulator on bone growth. We are to synthesize HA/Si via sol-gel route, followed by adding zinc oxide covering with chitosan to form a composite material.
The XRD patterns show that HA phases can be obtained at 500oC for pH 0 condition, but for pH 9 at lower temperature of 300oC. It can be seen the appearance of extra CaO and B-TCP phase. Concerning tensile strength, the value of monolithic HA derived from the sol-gel processing at pH 9 was much higher than that at pH 0, and higer pH value condition of sol could prevent diameter tensil strength decreased which was caused by silicon addition . There was no significant effect of ZnO addition on DTS value (p > 0.05) in the present study, however, enhancing an oriented growth of the HA phase on the (002) plane. The covering of chitosan didn’t effect the diameter tensile strength but bioactive, SEM microstructure shows more apatite formed on the chitosan surface after immersed in Hank’s solution for 30 days.

並列關鍵字

sol gel ； silicon ； hydroxyapatite ； Chitosan

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