蜆精對四氯化碳誘發大白鼠急性肝臟損傷之影響

Effect of Freshwater Clam Extract on Acute Liver Injury Induced by Carbon Tetrachloride in Rats

江孟燦(Meng-Tsan Chiang) ； 陳長堅(Charnng-Jian Chen) ； 姚賢宗(Hsein-Tsung Yao)

《臺灣農業化學與食品科學》 43卷6期 (2005/12) Pp. 402-409

https://doi.org/10.6578/TJACFS.2005.051

摘要

本研究探討蜆精對由四氯化碳所引起之大白鼠急性肝損傷之影響。以六週齡雄性Wistar大白鼠為試驗動物，試驗共分成三組，分別是(l)控制組(saline only)(2)蜆精組(20% w/v in saline)及(3)肝醣組(2% w/v in saline)。大白鼠禁食14小時後抽取尾部靜脈血液測定血漿轉胺酶活性及TBARS(thiobarbituric acid reactive substance)(0 min)值後，再經腹腔注射四氯化碳(0.2 mL/kg B.W in olive oil)並灌食樣品(4 mL/kg B.W in saline)，於灌食後第12、24小時抽血並再灌食相同試驗樣品，於第48小時將動物犧牲後立即取出肝臟。結果發現，蜆精及肝醣組在試驗結束後會顯著降低血漿膽紅素及TBARS濃度，且有較高之肝臟還原型麩胱甘肽含量，但肝臟TBARS值、麩胱甘肽過氧化酶及麩胱甘肽轉硫酶活性並無明顯改變。此外，三組間血漿轉胺酶活性亦無統計上差異。綜合以上結果顯示，蜆精可能藉由降低肝臟麩胱甘肽的耗損進而改善由四氯化碳所引起之急性肝損傷，而其中肝醣可能扮演重要的角色。

關鍵字

蜆精 ； 肝醣 ； 肝損傷 ； 四氯化碳 ； 大白鼠

並列摘要

To investigate the effect of freshwater clam extract on acute liver injury induced by carbon tetrachloride (CCI4), male Wistar rats were used as experimental animals and divided into three groups. They are (1) Saline group, (2) Freshwater clam extract group (20% w/v in saline) and (3) Glycogen group (2% w/v in saline). After 14 hours of fasting, the blood was sampled with heparin from tail vein to measure the plasma alanine aminotransaminase (ALT) and aspartate aminotransaminase (AST) activities and the TBARS value. The animals were then injected with Cd4 (0.2 mL/kg B.W., i.p) and were administered with the tested materials (4 mL/kg B.W.) using a gastric tube. After the 12 and the 24 hours of the first dosage, blood samples were collected from the tail vein and the freshwater clam extract and glycogen were readministered. After 48 hours of first dosage, rats were sacrificed. The results showed that rats dosed with freshwater clam extract or glycogen had lowered plasma total bilirubin and TBARS levels at the end of the experiment. Although higher hepatic reduced glutathione (GSH) content and GSH/GSSG ratio were observed in the freshwater clam extract group, liver TBARS value and glutathione peroxidase and glutathione-S-transferase activities were not affected by the freshwater clam extract and glycogen administration. Plasma AST and ALT activities were similar among the three dietary groups during the experimental periods. Results of this study suggest that freshwater clam extract might ameliorate acute liver injury induced by Cd4 in rats and glycogen might play an important role.

並列關鍵字

Freshwater clam extract ； Glycogen ； Liver injury ； Carbon tetrachloride ； Rats