台灣食用鳳梨癒合組織誘導與植株再生系統之建立

Establishment of Callus Induction and Shoot Regeneration System from Axillary Bud on Taiwanese Edible Pineapples

張雅雁(Ya-Yen Zhang) ； 許惠娟(Huei-Juan Hsu) ； 黃文理(Wen-Lii Huang)

《臺灣農業化學與食品科學》 46卷2期 (2008/04) Pp. 49-56

https://doi.org/10.6578/TJACFS.2008.007

摘要

本研究選取台農17號(TNG-17)和台農20號(TNG-20)兩種台灣食用鳳梨(Ananas comosus L. Merrill)品種冠芽之腋芽，於添加不同濃度BA與NAA之MS基礎培養基中進行培養，觀察癒合組織誘導與植株再生情形。結果顯示當鳳梨腋芽培養在含NAA培養基中，兩品種皆有明顯應合組織形成，但結構較鬆形也蝗易褐化。若將腋芽培養在同時添加BA與NAA培養基中，可誘發擬球體(protocom-like body, PLB)組織，將上述癒合組織與PLB移到MSB4N4培養基後一個月，TNG-20易誘發體胚發生，而TNG-17則有同時進行體胚發生與器官發生的趨勢，進一步培養後，兩者均可順利發育成完整植株。此外，結果也發現TNG-17癒合組織中有大量且明顯的澱粉粒分佈，推測可能與細胞分化有關，此為鳳梨組織培養中首度之報導，值得進一步深入研究。

關鍵字

食用鳳梨 ； 細胞分化 ； 體胚發生 ； 器官發生 ； 澱粉

並列摘要

Two cultivars, Tainung 17 (TNG-17) and Tainung 20 (TNG-20), of Taiwanese edible pineapple (Ananas comosus L. Merrill) were used in Ibis study. The axillary buds from pineapple crown grown in the field were selected and inoculated on MS basal medium supplement with different combinations of NAA and BA. It showed that the callus could be induced when NAA supplemented in the medium, However, the texture is loose and browning. Besides, protocorm-like body (PLB) formed when the explants were inoculated on the medium containing BA and NAA. After being transferred the callus and PLB onto MSB4N4 medium, somatic embryogenesis wilt be mainly observed in TNG-20. However, somatic embryogenesis and organogenesis are observed in TNG-17 during cell differentiation. We have developed efficient methods for plant regeneration, via both embryogenesis and organogenesis on Taiwanese edible pineapple. In addition, we also found abundance of starch granules spread in TNG-17 callus. It is the first discovery in pineapple tissue culture. Further studies is necessary to illuminate the possible roles of starch accumulation during callus induction and cell differentiation.

並列關鍵字

Ananas comosus ； Cell differentiation ； Embryogenesis ； Organogenesis, Starch