豬腦之脂肪酸組成與脯胺醯內肽酶抑制活性之研究

The Study of Fatty Acid Composition and Prolyl Endopeptidase Inhibitory Activity of Porcine Brain

柳育澤(Yu-Tse Liu) ； 鄭富元(Fu-Yuan Cheng) ； 倪千祥(Chien-Hsiang NI) ； 萬添春(Tien-Chun Wan) ； 陳志銘(Chi-Ming Chen) ； 林亮全(Liang-Chuan Lin)

《臺灣農業化學與食品科學》 54卷3期 (2016/06) Pp. 135-142

摘要

本研究乃以豬隻屠宰場之副產物－豬腦為原料，分析其一般成分與胺基酸組成，並區分成大腦皮質、大腦白質、小腦、腦幹及海馬迴等部位，再分析其飽和脂肪酸與不飽和脂肪酸組成及脯胺醯內肽酶（Prolyl Endopeptidase, PEP）抑制能力，藉以探討豬腦萃取為PEP抑制劑之可行性。結果顯示，豬腦去掉水分後脂肪與蛋白質各約有50%，胺基酸之組成以疏水性胺基酸為主；而豬腦之脂肪酸組成（濕重），分別為單元不飽和33.85 mg/100g、多元不飽和19.6 mg/100g及飽和46.46 mg/100g，其中飽和脂肪酸部份以硬脂酸（Stearicacid）含量最高（25.11 ± 0.09 mg/100g），而不飽和脂肪酸部分以油酸（Oleic acid）含量最高（30.05 ± 0.96 mg/100g），及二十二碳六烯酸（DHA）也含有7.18 ± 0.52 mg/100g。豬腦各部位之脂肪酸含量隨部位不同而有差異，發現DHA在大腦白質之含量顯著最高（10.62 ± 0.32 mg/100g），而腦幹之油酸含量顯著最高（40.70 ± 0.08 mg/100g），此結果也反應在PEP抑制活性，以腦幹具最佳之PEP抑制活性（IC_(50)=5.5 ± 0.82 mg/mL）。綜觀上述，豬腦之脂肪酸可能萃取出具有一定效果之PEP改善劑，具有開發作為減緩神經退化性疾病的功能性保健食品之潛力。

關鍵字

豬腦 ； 脂肪酸 ； 脯胺醯內肽酶活性

並列摘要

The purpose of this study was to investigate the ProlylEndopeptidase (PEP) activity inhibitor for porcine brain and which was collected form slaughter house. Proximate composition, amino acids, saturated fatty acids, unsaturated fatty acids and PEP inhibitory activity in various region of porcine brain including cerebrum cortex (CRC), cerebrum white matter (CRW), cerebellum (CL), brain stem (BS) and hippocampus (H) were carried out in this study. The results indicated that protein and lipid were about 50% while remove moisture in the porcine brain individually, fatty acid composition were monounsaturated 33.85 mg/100g, polyunsaturated 19.6 mg/100g, and saturated 46.46 mg/100g individually. Stearic acid has the highest amount (25.11 ± 0.09 mg/100g) in unsaturated, and the highest amount for unsaturated fatty acids is oleic acid (30.05 ± 0.96 mg/100g), moreover, DHA has 7.18 ± 0.52 mg/100g in porcine brain. Different fatty acids content found in various region of porcine brain, DHA was found the highest (10.62 ± 0.32 mg/100g) in CRW, and BS has the highest amount for oleic acid (40.70 ± 0.08 mg/100g), this result has positive effect on PEP inhibitory activity, while BS has the best ability (IC_(50) = 5.5 ± 0.82 mg/mL) was higher than other regions (p ＜ 0.05). These results suggested that porcine brain had high potential to research the PEP inhibitory activity form fatty acids and maybe used in neurodegenerative disease.

並列關鍵字

Porcine brain ； Fatty acid ； Prolyl Endopeptidase activity