食品中蘆薈素及蘆薈大黃素檢驗方法評估

Evaluation of the Analysis Method of Aloin and Aloe Emodin in Food

沈盈如(Ying-Ru Shen) ； 古璦寧(Ai-Ning Ku) ； 吳白玟(Pai-Wen Wu) ； 廖家鼎(Chia-Ding Liao) ； 高雅敏(Ya-Min Kao) ； 陳惠芳(Hwei-Fang Cheng)

《臺灣農業化學與食品科學》 54卷6期 (2016/12) Pp. 259-268

摘要

蘆薈具芳香特性、苦味及藥理活性，廣泛作為食品、飲料、膳食補充品及製藥等成分，有研究顯示未經脫色處理之蘆薈全葉萃取物會引起大鼠大腸癌和造成大鼠大腸、小腸、胃及淋巴結等增生之風險，基於安全考量，含蘆薈產品需進行適當品質控管。目前國內外對於相關產品訂定之規範或建議主要針對蘆薈素(Aloin)含量，考量蘆薈大黃素(Aloe Emodin)為其代謝物之一，故本研究選取Aloin A、Aloin B及Aloe Emodin等3化合物作為目標分析物，檢體方面選用「膠囊粉劑」、「優格」及「飲料」等3種常見型態作為評估基質，並同時進行液相層析儀搭配光二極體陣列檢出器(liquid chromatography-photodiode array detector, LC-PDA)及液相層析串聯質譜儀(liquid chromatography-tandem mass spectrometry, LC-MS/MS)等2種儀器分析，目的為建立簡便、適用範圍廣及能符合規範之檢驗方法供不同需求選擇使用。結果顯示，固態可檢測範圍介於0.1-1,000 μg/g(LC-PDA：10-1000 μg/g；LC-MS/MS：0.1-10 μg/g)；半固態及液態可檢測範圍則介於0.01-10 μg/g(LC-PDA：0.2-10 μg/g；LC-MS/MS：0.01-2 μg/g)；整體來說，回收率介於61-121%、變異係數皆＜16%，試驗結果理想。本研究建立之檢驗方法可同步進行Aloin A、Aloin B及Aloe Emodin等3品項分析，適用不同類型食品，並可因應需求選擇使用LC-PDA或LC-MS/MS進行分析。

關鍵字

蘆薈素 ； 蘆薈大黃素 ； LC-PDA ； LC-MS/MS ； 蘆薈產品

並列摘要

Aloe is widely used composition to manufacture food, beverages, dietary supplements or pharmaceuticals because of its aromatic properties, bitter taste, and pharmacological activities. Some studies showed that nondecolorized whole aloe leaf extract caused cancers of the large intestine in rats and also caused the risk of hyperplasia of the large intestine, small intestine, stomach, and lymph nodes in rats. For the safety reason that aloe commercial products should be control its quality. Concentration of Aloin is as the requirement of specification or recommendations for products of aloe in both domestically and abroad so far. Consider Aloe Emodin is metabolism of Aloin, so Aloin A, Aloin B and Aloe Emodin were selected in this study. In order to develop a method which can meet simple, widely use, and the requirement of specifications, the study was developed to determine the 3 compounds in 3 common products (capsule powder, yoghurt and beverage) with liquid chromatography-photodiode array detector (LC-PDA) or liquid chromatography-tandem mass spectrometry (LC-MS/MS) for options. The linear range of solid sample was from 0.1-1,000 μg/g (LC-PDA: 10-1,000 μg/g; LC-MS/MS: 0.1-10 μg/g) while semisolid sample and liquid sample were from 0.01-10 μg/ g (LC-PDA: 0.2-10 μg/g; LC-MS/MS: 0.01-2 μg/g). For overall results, this study had satisfied recovery (61- 121%) and well repeatability (＜ 16%). The developed method has been applied multiple 3 compounds and suitable for various products with LC-PDA or LC-MS/MS for options.

並列關鍵字

Aloin ； Aloe Emodin ； LC-PDA ； LC-MS/MS ； Products of aloe