不同乾燥處理之小果油茶樹葉甲醇萃取物抗氧化活性之探討

Antioxidative Activity of Methanol Extract from Camellia Leaves with Different Drying Methods

蘇品嘉(Pin-Chia Su) ； 徐錫樑(Shyi-Liang Shyu)

《臺灣農業化學與食品科學》 55卷1期 (2017/02) Pp. 59-65

https://doi.org/10.6578/TJACFS.2017.006

摘要

本研究主要探討小果油茶樹葉經由不同乾燥方法(冷凍乾燥、低溫低濕乾燥和熱風乾燥)處理，探討其 萃取物之抗氧化活性。結果顯示油茶樹葉以不同乾燥方法處理(乾燥物以甲醇萃取)，其萃取率、抗氧化活性大小依序為冷凍乾燥＞低溫低濕乾燥＞熱風乾燥。其中小果油茶樹葉經冷凍乾燥法處理具有最好的抗氧化活性，其總酚及類黃酮分別為190.35 GAE mg/g d.m.及87.87 RE mg/g d.m.。當樣品濃度為100 ppm時，冷凍乾燥油茶樹葉甲醇萃取物之DPPH自由基清除能力及三價鐵還原能力分別為94.06%及OD 700 nm = 1.55，其效果等同於100 ppm BHT；濃度在1,000 ppm時，亞鐵離子螯合能力為30%。冷凍乾燥油茶樹葉甲醇萃取物的主要酚類化合物為芸香苷、兒茶素和綠原酸。小果油茶樹葉中富含抗氧化成分，因此本研究認為冷凍乾燥油茶樹葉甲醇萃取物應可作為天然抗氧化劑及具有當作加工產品原料之潛力。

關鍵字

小果油茶樹葉 ； 乾燥 ； 抗氧化活性 ； 氧化安定性

並列摘要

This study was aiming to investigate the antioxidative capacity of camellia leaves (Camellia brevistyla) and its development potential into processed food products. The antioxidative activity of extract obtained from camellia leaves dried with different drying methods (including hot air, HD; low temperature and low humidity, LD; and freeze drying, FD) were studied. Results showed that the order of extraction yield was FD ＞ LD ＞ HD. The freeze drying camellia leaves methanol extract (FCME) had the highest total phenolic content and total flavonoid content which was 190.35 GAE mg/g d.m. and 87.87 RE mg/g d.m., respectively. When the sample concentration was at 100 ppm, the DPPH radical scavenging and reducing power of FCME was 94.06% and OD 700 nm = 1.55, respectively, which was similer to BHT. The ferrous ion chelating capacity of FCME could reach to 30% as the sample concentration was at 1,000 ppm. The major phenolic compounds of the FCME were rutin, catechin and chlorogenic acid. Camellia leaves were rich in antioxidative components. Therefore, this study suggested that the FCME could be used as a natural antioxidant and potential material for food processing.

並列關鍵字

Camellia leaves ； Drying ； Antioxidative activity ； Oxidation stability