培養基質對微生物降解柴油氣相層析分析之干擾與消除

The Interference of Medium on Gas Chromatographic Analysis of Diesel in Microbial Degradation and its Elimination

柯琪峰(Chi-Fong Ko) ； 蘇強升(Chiang-Sheng Su) ； 黃詠仁(Yung-Jen Huang) ； 施養信(Yang-hsin Shih)

《臺灣農業化學與食品科學》 59卷2期 (2021/06) Pp. 64-69

https://doi.org/10.6578/TJACFS.202106_59(2).0005

摘要

本研究旨在評估經過柴油馴化與降解試驗，所篩選出BS、NO.7及EM1（堆肥菌之混合菌株）等具有降解柴油化合物潛力之菌株。為了進一步應用生物強化的方式進行柴油污染場址整治作業，需要大量且廉價地生產製造柴油降解菌株。利用黃豆粉作為枯草桿菌的培養基質，以及枯草桿菌菌粉生產過程中額外添加賦形劑，除了可以增加菌粉穩定性及其保存期限外，經過生物固定化作用後，也可以增加其對於柴油化合物的降解能力。試驗結果指出，添加黃豆粉與賦形劑，會造成GC/FID氣相層析圖譜出現干擾訊號，導致柴油化合物的定量分析受到影響。本研究發現，該干擾訊號主要來自菌粉培養基原料（黃豆粉）中的有機成分。經水相柴油降解試驗以及在土壤中進行長時間的降解試驗，進一步以GC/FID分析發現，黃豆粉培養枯草桿菌的菌粉使柴油之總碳氫化合物訊號明顯降低，證實了菌粉中所含黃豆粉及賦形劑等添加成分，均能夠被微生物利用而減少。最後的結果也證明，以黃豆粉生產培養菌粉型態的枯草桿菌在柴油汙染場址生物修復應用上具有相當的潛力。

關鍵字

生物修復 ； 柴油降解 ； 枯草桿菌 ； 黃豆粉 ； 氣相層析

並列摘要

The purpose of this study is to evaluate the strains that have the potential to degrade diesel compounds, such as BS, NO.7 and EM1 (the mixtures of bacteria), which have been screened after diesel domestication and degradation tests. For further application of bioenhancement and bioremediation of diesel-contaminated sites, these diesel-degrading strains need to be produced in large scales inexpensively. The use of soybean flour as the culture medium of Bacillus sp. and the addition of excipients during the production of bacterial powder can not only increase the stability of the bacterial powder and its shelf life, but also increase its degradation of diesel compounds after immobilization. The results indicated that the addition of soybean flour and excipients generated interference signals in the GC/FID analysis, which affected the quantification of diesel compounds. In this study, we found that the interference signals mainly come from the organic components in the soy flour, which is the culture medium used for the production of bacterial powder. After the diesel degradation test in water and the long-term degradation experiment in soil, the results of GC/FID analysis showed that the total petroleum hydrocarbon (TPH) signal of diesel with the bacterial powder of Bacillus sp. was significantly reduced. It was confirmed that the soybean flour and excipients contained in the bacterial powder can be utilized by microorganisms. Finally, it has been demonstrated that the use of soybean flour to produce bacterial powder of Bacillus sp. has considerable potential for application in bioremediation of diesel contaminated sites.

並列關鍵字

Bioremediation ； Diesel degradation ； Bacillus sp. ； Soybean Flour ； GC/FID