In Vitro Antibiotic Susceptibility of Lactobacilli Isolated from Commercial Products Containing Active Lactobacilli

我們調查市面上數種口服活性乳酸菌製劑對於在臨床上常用的抗生素的最低抑菌濃度值(minimum inhibitory concentration, MIC)。我們隨機抽四種上市的活性乳酸菌制劑産品，含有Lactobacillus acidophilus, Bifidobacteria infantis等菌，取其粉末加以培養，並對各菌落用API50 CH測試組及RAPID　ID32A測試組，得知産品包含有B. infantis, L. acidophilus, Lactobacillus casei等菌株，將B. infantis及L. acidophilus, L.casei等株分別置入含硫甘醇酸鹽培養管，將此種培養基液稀釋，定量接種於Brucella blood agar-MIC培養基上，採用瓊脂稀釋法。培養基內分別含有不同濃度的amoxicillin, cephalothin, gentamicin, vancomycin, erythromycin, rifampin, tetracycline, 及penicillin G, clindamycin, chloramphenicol, cefmetazole, metronidazole, ampicillin/sulbactum, cefoxtin等臨床常用的抗生素用藥，檢測各抗生素不同濃度中的生長情況，決定breakpoint濃度，並判斷對各抗生素的感受性。得知amoxicillin, ampicillin/sulbactum及penicillin-G的MIC值對上述三菌種皆≦2μg/ml，L. casei對cephalothin, vancomycin, erythromycin, clindamycin, metronidazole, cefmetaxole, cefoxtin的MIC值皆＞32μg/ml，L. casei在本實驗是對所有測試的抗生素中最具抗藥性的一種益生菌。在得知各個活性乳酸菌制劑對各種抗生素的抗生後，選擇特定抗藥性高的活性乳酸菌制劑來預防抗生素治療時副作用的産生，可能可能可以應用在兒科領域中，預防病童接受抗生素治療後造成的腹瀉症狀。

關鍵字

乳酸菌；抗生素感受性；乳酸菌製劑

並列摘要

To survey the antibiotic susceptibility patterns of some commercially available Lactobacillus, we collected four commercial products that contain active Lactobacillus. We incubated individual product and identified these colonies by the methods of API50 CH test kit and RAPID ID 32A kit. Strains of Streptococcus thermophilus, Bifido bacterium infantis, Lactobacillus acidophilus and Lacto bacillus casei were collected. By agar dilution method, each identified strain was inoculated to Brucella blood agar-MIC plates. Each plate contained one of the following antibiotics with d/erent concentrations: amoxicillin, cephalothin, gentamicin, vancomycin, erythromycin, rifampin, tetracyclin and penicillin G, clindamycin, chloramphenicol, cefmetazole, metronidazole, ampicillin/sulbactum, cefoxtin, etc. After incubation, the growth condition of each Brucella blood agar-MIC plate was observed and the breakpoint of each antibiotic to different lactobacillus products determined. The MICs of amoxicillin, ampicillin/sulbactum and penicillin-G to all identified strains were ≦2μg/ml and those of vancomycin, clindamycin, erythromycin, metronidazole, cefmetazole and cefoxtin for L. casei were ＞32μg/ml. L. casei was more resistant to all the testing antibiotics than the other strains. According to the MICs of the above antibiotics, proper active lactobacillus products could be chosen to prevent antibiotic-associated diarrhea in the pediatric field.

並列關鍵字

antibiotic susceptibility ； Lactobacillus ； Bifidobacteria infantis ； minimum inhibitory concentration

國際替代計量

In Vitro Antibiotic Susceptibility of Lactobacilli Isolated from Commercial Products Containing Active Lactobacilli

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