The Technique for Measurement of Cell Adhesion Force

細胞黏著力量測技術

李真甄(Chen-Chen Lee) ； 吳佳慶(Chia-Ching Wu) ； 蘇芳慶(Fong-Chin Su)

《Journal of Medical and Biological Engineering》 24卷1期 (2004/03) Pp. 51-56

摘要

細胞接觸基質或材料時，細胞首先發生貼附及黏著的現象，這個現象會決定細胞在基材上的生理表現，進而影響細胞所能發揮的特有功能，因此目前已有細胞生理學、生物化學等領域的學者藉由評估細胞的黏著能力，來評斷細胞的表現，近年來也有力學等領域的學者藉由定量細胞黏著力來評估細胞黏著能力，故本研究藉由設計一套可量測細胞黏著力的機構來定量細胞黏著能力。機構中採用原子力顯微鏡探針上的懸桿為施力為施力設備，藉由細胞以等速逼近懸桿，使懸桿對細胞施加剪力並將細胞從基材上刮下，再分析懸桿的偏移量求出探針對細胞的施力，藉此求得細胞的黏著力量。完成機構測試後，並利用此機構觀察NIH/3T3纖維母細胞在不同實驗溶液下，細胞黏著力量的改變，結果發現細胞在相同溫度下於Dulbeccos Modification of Eagles Medium （DMEM，無酚紅）培養基加10%胎牛血清(Fetal Bovine Serum, FBS)中的黏著現最佳，其次為在DMEM（無酚紅）中，而在無機鹽溶液(Phospate-Buffered Saline, PBS)中的表現則最差。

關鍵字

細胞黏著力 ； 微小懸桿 ； NIH/3T3纖維母細胞

並列摘要

After cells attach the substrata, cells begin to spread and form an organized actin cytoskeleton and complex transmembrane signaling regions, then cells start to express their functions, such as proliferation, differentiation and so on. Therefore, cell adhesion is very important. Recently, a number of studies have investigated cell adhesion from the viewpoint of biochemistry, materials, and mechanics. In this study, we observed the adhesion from mechanical point of view. The objective of this study was to develop a cytodetachment technique to quantitatively measure the adhesive force between cells and substrata. A holder was designed which can hold the probe of the AFM (atomic force microscope) probe, which was integrated to a laser tweezers workstation. A detach rig was setting, which is consisted of the probe of AFM, an AFM probe holder, and a cantilever arm mounted on the laser tweezers workstation. NIH/3T3 fibroblast seed on non-coated glass microscope slide, and test in different test mediums, such as Dulbeccos modification of eagles medium (DMEM)+10% fetal bovine serum (FBS) versus DMEM and Phospate-Buffered Saline (PBS). Detach NIH/3T3 fibroblast at room temperature, 25℃. Cell adhesion force of NIH/3T3 fibroblast in different test mediums. A novel cell-detachment apparatus to measure the adhesion force of a single cell was developed to incorporate into an optical tweezers workstation. The results demonstrate that a greater force is required to detach cells in DMEM+10% FBS versus DMEM and Phospate-Buffered Saline (PBS). In this study, we set a rig to quantify cell adhesion force. And the result of this study showed that adhesion force of NIH/3T3 fibroblast in DMEM+10% FBS is greater then cells in DMEM and PBS.

並列關鍵字

Cell adhesion force ； Microcantilever ； NIH/3T3 fibroblast