- 期刊
Evaluation of Human Bone Marrow Mesenchymal Stem Cells Seeded into Composite Scaffolds and Cultured in a Dynamic Culture System for Neocartilage Regeneration in vitro
並列摘要
Homan hone marrow mesenchymal stein cells (hBMSCs) were seeded into two types of scaffolds, including blended polymers of PLGA 50/50 and PLLA modified by type 11 collagen (BC/11) and complexes of chitosan and gelatin (CG). The seeded scaffolds were incubated in culture medium with TGF-β3 for 23 days under static or dynamic culture condition. The cell number, amount of matrix, cell morphology and cell differentiation in constructs were determined. The cell number for each scaffold was higher when TGF-β3 was added into the culture medium. Cell numbers in CG scaffolds were higher than those in BCII scaffolds. Cell proliferation increased under dynamic culture condition. Many differentiated cells as identified by anti S-100 staining were observed when TGP-β3 was employed. The difference in materials of the two scaffolds had no apparent effect on TGF β3 induced hBMSCs transformation into differentiated cells The dynamic culture system promoted cell proliferation, hut not cell differentiation.
被引用紀錄
延伸閱讀
- 胡家僖（2011）。The Effect of Articular cartilage fragments and synovial membranes on Chondrogenesis of Bone Marrow- Derived Mesenchymal Stem Cells〔碩士論文，國立臺北科技大學〕。華藝線上圖書館。https://doi.org/10.6841/NTUT.2011.00127
- Kim, D. H., Kim, T. W., Lee, J. D., Shin, K. K., Jung, J. S., Hwang, K. H., Lee, J. K., Park, H. C., & Yoon, S. Y. (2013). Preparation and In Vitro and In Vivo Performance of Magnesium Ion Substituted Biphasic Calcium Phosphate Spherical Microscaffolds as Human Adipose Tissue-Derived Mesenchymal Stem Cell Microcarriers. Journal of Nanomaterials, 2013(), 336-344-438. https://doi.org/10.1155/2013/762381
- 夏毅然、張燕清、彭志網、史中（2006）。Effects and Possible Mechanisms of Stem Cells and Biomaterials on Repair/Regeneration of Critical-sized Femora Bone Defects。中華民國口腔顎面外科學會雜誌，17(2)，73-78。https://doi.org/10.7104/CJOMS.200609.0073
- Hong, M. X. (2015). Investigating the role of human bone marrows-mesenchymal stem cells for the initialization and metastasis of lung cancer stem cells: a systems biology approach [master's thesis, National Tsing Hua University]. Airiti Library. https://doi.org/10.6843/NTHU.2015.00539
- 史中、夏毅然、彭志綱、張燕清（2007）。Effects of Marrow Mesenchymal Stem Cells and PLA/PGA on Cellular Protein Expression during Regeneration of Large Bone Defects。中華民國口腔顎面外科學會雜誌，18(2)，81-88。https://doi.org/10.7104/CJOMS.200709.0081